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Antibacterial and Biocompatible Cross-Linked Waterborne Polyurethanes Containing Gemini Quaternary Ammonium Salts
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-01-02 00:00:00 , DOI: 10.1021/acs.biomac.7b01016
Yi Zhang 1, 2 , Xueling He 1, 3 , Mingming Ding 1 , Wei He 1 , Jiehua Li 1 , Jianshu Li 1 , Hong Tan 1
Affiliation  

A cross-linked waterborne polyurethane (CPTMGPU) with long-term stability was developed from poly(ethylene glycol) (PEG), polyoxytetramethylene glycol (PTMG), isophorone diisocyanate (IPDI), l-lysine, and its derivative diamine consisting of gemini quaternary ammonium salt (GQAS), using ethylene glycol diglycidyl ether (EGDE) as a cross-linker. Weight loss test, X-ray photoelectron spectroscopy (XPS) measurements, and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) were performed to prove the surface structure and stability of these CPTMGPU films. Furthermore, the GQAS-bearing CPTMGPUs show repeatable contact-active antibacterial efficacy against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria and do not show any inhibition effect against fibroblasts in vitro. After subcutaneous implantation in rats, the CPTMGPU films manifest good biocompatibility in vivo, despite the presence of a typical foreign body reaction toward surrounding tissues and mild systematic inflammation reaction that could be eliminated after a short implantation period, as demonstrated by histology and immunohistochemistry combined with interleukin (IL)-1β, IL-4, IL-6, IL-10, and TNF-α analysis though enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (qRT-PCR). Therefore, these cross-linked waterborne polyurethanes hold great promise for antibacterial applications in vivo.

中文翻译:

含有双子座季铵盐的抗菌和生物相容的交联水性聚氨酯

由聚乙二醇(PEG),聚氧丁二醇(PTMG),异佛尔酮二异氰酸酯(IPDI),1-赖氨酸及其由双子座季铵盐组成的衍生物二胺开发出具有长期稳定性的交联水性聚氨酯(CPTMGPU)铵盐(GQAS),使用乙二醇二缩水甘油醚(EGDE)作为交联剂。进行了重量损失测试,X射线光电子能谱(XPS)测量和衰减全反射-傅立叶变换红外光谱(ATR-FTIR),以证明这些CPTMGPU膜的表面结构和稳定性。此外,带有GQAS的CPTMGPU对革兰氏阳性金黄色葡萄球菌S. aureus)和革兰氏阴性菌均表现出可重复的接触活性抗菌功效大肠杆菌E. coli)细菌,在体外对成纤维细胞没有任何抑制作用。大鼠皮下植入后,CPTMGPU膜表现出良好的体内生物相容性,尽管存在典型的异物对周围组织的反应和轻度的系统性炎症反应,这些现象可在短时间植入后消除,如组织学和免疫组织化学与通过酶联免疫吸附测定(ELISA)和实时定量聚合酶链反应(qRT-PCR)分析白介素(IL)-1β,IL-4,IL-6,IL-10和TNF-α。因此,这些交联的水性聚氨酯在体内抗菌应用方面具有广阔的前景。
更新日期:2018-01-02
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