Electrical Neural Stimulation and Simultaneous in Vivo Monitoring with Transparent Graphene Electrode Arrays Implanted in GCaMP6f Mice,ACS Nano

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Electrical Neural Stimulation and Simultaneous in Vivo Monitoring with Transparent Graphene Electrode Arrays Implanted in GCaMP6f Mice
ACS Nano ( IF 15.8 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1021/acsnano.7b04321
Dong-Wook Park ₁ , Jared P. Ness , Sarah K. Brodnick , Corinne Esquibel , Joseph Novello , Farid Atry ₂ , Dong-Hyun Baek , Hyungsoo Kim , Jihye Bong , Kyle I. Swanson ₃ , Aaron J. Suminski , Kevin J. Otto , Ramin Pashaie ₂ , Justin C. Williams , Zhenqiang Ma

Affiliation

Electrical stimulation using implantable electrodes is widely used to treat various neuronal disorders such as Parkinson’s disease and epilepsy and is a widely used research tool in neuroscience studies. However, to date, devices that help better understand the mechanisms of electrical stimulation in neural tissues have been limited to opaque neural electrodes. Imaging spatiotemporal neural responses to electrical stimulation with minimal artifact could allow for various studies that are impossible with existing opaque electrodes. Here, we demonstrate electrical brain stimulation and simultaneous optical monitoring of the underlying neural tissues using carbon-based, fully transparent graphene electrodes implanted in GCaMP6f mice. Fluorescence imaging of neural activity for varying electrical stimulation parameters was conducted with minimal image artifact through transparent graphene electrodes. In addition, full-field imaging of electrical stimulation verified more efficient neural activation with cathode leading stimulation compared to anode leading stimulation. We have characterized the charge density limitation of capacitive four-layer graphene electrodes as 116.07–174.10 μC/cm² based on electrochemical impedance spectroscopy, cyclic voltammetry, failure bench testing, and in vivo testing. This study demonstrates the transparent ability of graphene neural electrodes and provides a method to further increase understanding and potentially improve therapeutic electrical stimulation in the central and peripheral nervous systems.

中文翻译：

用透明石墨烯电极阵列植入GCaMP6f小鼠体内进行电神经刺激和同时体内监测

使用植入式电极的电刺激被广泛用于治疗各种神经元疾病，如帕金森氏病和癫痫，并且是神经科学研究中广泛使用的研究工具。但是，迄今为止，有助于更好地理解神经组织中电刺激机制的设备仅限于不透明的神经电极。对电刺激的时空神经反应进行成像，使伪影最少，可以进行现有的不透明电极无法进行的各种研究。在这里，我们演示了使用植入GCaMP6f小鼠中的基于碳的完全透明石墨烯电极对脑神经进行刺激并同时对基础神经组织进行光学监控。通过透明的石墨烯电极以最小的图像伪影进行了针对各种电刺激参数的神经活动的荧光成像。此外，与阳极引导刺激相比，电刺激的全场成像证明了阴极引导刺激能更有效地激活神经。我们已经将电容性四层石墨烯电极的电荷密度限制定为116.07–174.10μC/ cm²基于电化学阻抗谱，循环伏安法，失效基准测试和体内测试。这项研究证明了石墨烯神经电极的透明能力，并提供了一种进一步增进了解并潜在改善中枢神经系统和周围神经系统治疗性电刺激的方法。

更新日期：2018-01-08

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