当前位置: X-MOL 学术ACS Chem. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Protein Inhibitors of CRISPR-Cas9
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2018-01-17 00:00:00 , DOI: 10.1021/acschembio.7b00831
Joseph Bondy-Denomy 1
Affiliation  

Bacteria are under constant predation from viruses, called bacteriophages (phages). This threat has driven the evolution of multiple defense systems, including the CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR associated genes) immune pathway. Phages are not passive bystanders in their CRISPR-mediated demise, however, as many have developed potent protein inhibitors of the bacterial adaptive immune system. Here, I review the work that led to the discovery of many distinct “anti-CRISPR” proteins. Furthermore, I outline how understanding their mechanisms of action has provided a suite of specific and high-affinity reagents to modulate and study CRISPR-Cas applications.

中文翻译:


CRISPR-Cas9 的蛋白质抑制剂



细菌不断受到病毒(称为噬菌体)的捕食。这种威胁推动了多种防御系统的进化,包括 CRISPR-Cas(成簇的规则间隔短回文重复序列和 CRISPR 相关基因)免疫途径。然而,噬菌体并不是 CRISPR 介导的死亡的被动旁观者,因为许多噬菌体已经开发出细菌适应性免疫系统的有效蛋白质抑制剂。在这里,我回顾了导致许多独特“抗 CRISPR”蛋白发现的工作。此外,我概述了对它们的作用机制的理解如何提供了一套特异性和高亲和力的试剂来调节和研究 CRISPR-Cas 应用。
更新日期:2018-01-17
down
wechat
bug