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Labeling and identifying cell-specific proteomes in the mouse brain.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2018-02-01 , DOI: 10.1038/nbt.4056
Toke P Krogager 1 , Russell J Ernst 1 , Thomas S Elliott 1 , Laura Calo 2 , Václav Beránek 1 , Ernesto Ciabatti 1 , Maria Grazia Spillantini 2 , Marco Tripodi 1 , Michael H Hastings 1 , Jason W Chin 1
Affiliation  

We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.

中文翻译:

在小鼠大脑中标记和识别细胞特异性蛋白质组。

我们开发了一种方法来标记由分离的皮层、脑切片和活小鼠大脑中的特定细胞类型合成的蛋白质组。通过病毒介导的正交吡咯赖氨酰-tRNA 合成酶-tRNAXXX 对在感兴趣的细胞类型中的表达并提供具有化学句柄的非经典氨基酸,我们选择性地标记神经元或神经胶质蛋白质组。该方法能够从空间和基因定义的大脑区域识别蛋白质。
更新日期:2017-12-18
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