Thousands of long noncoding RNAs (lncRNAs) have been identified in eukaryotic genomes, many of which are expressed in spatially and temporally restricted patterns. Nonetheless, the roles of the majority of these transcripts are still unknown. One of the mechanisms by which lncRNAs function is through the modulation of chromatin states. To assess the functions of lncRNAs, we developed RNA-DamID, a novel approach that detects lncRNA–genome interactions in a cell-type-specific manner in vivo with high sensitivity and accuracy. Identifying the cell-type-specific genome occupancy of lncRNAs is vital to understanding their mechanisms of action in development and disease. We used RNA-DamID to investigate targeting of the lncRNAs in the Drosophila dosage-compensation complex (DCC) and show that initial targeting is cell-type specific.

中文翻译：

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RNA-DamID在染色质进入位点揭示roX RNA的细胞类型特异性结合

在真核基因组中已经鉴定出成千上万的长非编码RNA（lncRNA），其中许多以空间和时间限制的模式表达。尽管如此，大多数这些成绩单的作用仍是未知的。lncRNA发挥作用的机制之一是通过染色质状态的调节。为了评估lncRNA的功能，我们开发了RNA-DamID，这是一种新颖的方法，可以在体内以细胞类型特异性的方式检测lncRNA与基因组的相互作用，具有很高的灵敏度和准确性。鉴定lncRNA的细胞类型特异性基因组占有率对于了解其在发育和疾病中的作用机制至关重要。我们使用RNA-DamID来研究果蝇中lncRNA的靶向 剂量补偿复合物（DCC），并显示初始靶向是细胞类型特异性的。