Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation.,Advanced Science

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Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation.
Advanced Science ( IF 14.3 ) Pub Date : 2017-12-18 , DOI: 10.1002/advs.201700678
Jinhua Li _{1,

2,

3} , Jin Wen ₄ , Bin Li ₅ , Wan Li ₂ , Wei Qiao ₆ , Jie Shen _{1,

3} , Weihong Jin ₂ , Xinquan Jiang ₄ , Kelvin W K Yeung _{1,

3} , Paul K Chu ₂

Affiliation

Understanding cell-biomaterial interactions is critical for the control of cell fate for tissue engineering and regenerative medicine. Here, cerium oxide nanoparticles (CeONPs) are applied at different Ce4+/Ce3+ ratios (i.e., 0.46, 1.23, and 3.23) to titanium substrate surfaces by magnetron sputtering and vacuum annealing. Evaluation of the cytotoxicity of the modified surface to cultured rat bone marrow mesenchymal stem cells (BMSCs) reveals that the cytocompatibility and cell proliferation are proportional to the increases in Ce4+/Ce3+ ratio on titanium surface. The bone formation capability induced by these surface modified titanium alloys is evaluated by implanting various CeONP samples into the intramedullary cavity of rat femur for 8 weeks. New bone formation adjacent to the implant shows a close relationship to the surface Ce4+/Ce3+ ratio; higher Ce4+/Ce3+ ratio achieves better osseointegration. The mechanism of this in vivo outcome is explored by culturing rat BMSCs and RAW264.7 murine macrophages on CeONP samples for different durations. The improvement in osteogenic differentiation capability of BMSCs is directly proportional to the increased Ce4+/Ce3+ ratio on the titanium surface. Increases in the Ce4+/Ce3+ ratio also elevate the polarization of the M2 phenotype of RAW264.7 murine macrophages, particularly with respect to the healing-associated M2 percentage and anti-inflammatory cytokine secretion. The manipulation of valence states of CeONPs appears to provide an effective modulation of the osteogenic capability of stem cells and the M2 polarization of macrophages, resulting in favorable outcomes of new bone formation and osseointegration.

中文翻译：

钛表面氧化铈纳米颗粒的价态操纵用于调节细胞命运和骨形成。

了解细胞与生物材料的相互作用对于控制组织工程和再生医学的细胞命运至关重要。在这里，通过磁控溅射和真空退火，将不同 Ce4+/Ce3+ 比率（即 0.46、1.23 和 3.23）的氧化铈纳米粒子 (CeONP) 涂覆到钛基底表面。修饰表面对培养的大鼠骨髓间充质干细胞（BMSCs）的细胞毒性评估表明，细胞相容性和细胞增殖与钛表面Ce4+/Ce3+比率的增加成正比。通过将各种CeONP样品植入大鼠股骨髓内腔8周来评估这些表面改性钛合金诱导的骨形成能力。种植体附近的新骨形成与表面 Ce4+/Ce3+ 比率密切相关；较高的 Ce4+/Ce3+ 比率可实现更好的骨整合。通过在 CeONP 样品上培养大鼠 BMSC 和 RAW264.7 小鼠巨噬细胞不同时间来探索这种体内结果的机制。BMSCs成骨分化能力的提高与钛表面Ce4+/Ce3+比例的增加成正比。Ce4+/Ce3+ 比率的增加也会提高 RAW264.7 鼠巨噬细胞 M2 表型的极化，特别是在与愈合相关的 M2 百分比和抗炎细胞因子分泌方面。CeONPs 价态的操纵似乎可以有效调节干细胞的成骨能力和巨噬细胞的 M2 极化，从而产生新骨形成和骨整合的良好结果。

更新日期：2017-12-18

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