Bisphenol A (BPA), discovered to be an artificial estrogen, has been shown to leach from some containers and mediate oxidative damage to cells and tissues and to be involved in reproductive disorders, obesity, diabetes, and liver dysfunction. In the current study, we investigated the effects of oral chronic exposure to low dose of BPA (0.5 mg kg⁻¹) on the protein and phosphoprotein expression profiles in male Wistar rat liver using a gel-based proteomics approach based on two-dimensional gel electrophoresis followed by matrix-assisted laser desorption/ionization mass spectrometry identification. Our results showed that BPA exposure affected the levels of proteins and phosphoproteins involved in diverse biological processes associated with hepatotoxicity, fatty liver, and carcinoma. Moreover, we analyzed the effects of BPA on oxidative stress by assessing levels of malondialdehyde (MDA), a marker of lipid peroxidation, and reduced glutathione (GSH), a non-enzymatic antioxidant agent, in the liver. As expected BPA induced oxidative stress indicated by increased levels of MDA and decreased GSH content in the liver. In conclusion, chronic oral exposure of rats to BPA leads to increased oxidative stress in the liver and major alterations in the liver proteome and phosphoproteome, which may contribute to the pathophysiology of liver diseases.

已发现双酚A（BPA）是一种人造雌激素，已显示会从某些容器中浸出并介导对细胞和组织的氧化损伤，并参与生殖系统疾病，肥胖症，糖尿病和肝功能障碍。在当前的研究中，我们调查了口服长期暴露于低剂量BPA（0.5 mg kg ^-1）使用基于蛋白质组学的蛋白质组学方法，基于二维凝胶电泳，然后通过基质辅助激光解吸/电离质谱鉴定，对雄性Wistar大鼠肝脏中的蛋白质和磷蛋白表达谱进行分析。我们的结果表明，BPA暴露会影响与肝毒性，脂肪肝和癌相关的多种生物过程中涉及的蛋白质和磷蛋白的水平。此外，我们通过评估肝脏中脂质过氧化的标志物丙二醛（MDA）和非酶促抗氧化剂还原型谷胱甘肽（GSH）的水平，分析了BPA对氧化应激的影响。如预期的那样，肝脏中MDA含量升高和GSH含量降低表明BPA诱导了氧化应激。综上所述，