In the last few decades, flow cytometry has redefined the field of biology, exponentially enhancing our understanding of cells, immunology, and microbiology. Flow cytometry recently gave birth to flow virometry, a new way to detect, analyze, and characterize single viral particles. Detection of viruses by flow cytometry is possible due to improvements in current flow cytometers, calibration, and tuning methods. We summarize the recent birth and novel uses of flow virometry and the progressive evolution of this tool to advance the field of virology. We also discuss the various flow virometry methods used to identify and analyze viruses. We briefly summarize other applications of flow virometry, including: virus detection, quantification, population discrimination, and viral particles' antigenic properties. Finally, we summarize how viral sorting will allow further progress of flow virometry to relate viral surface characteristics to infectivity properties.

中文翻译：

---

流动病毒学作为研究病毒的工具

在过去的几十年里，流式细胞术重新定义了生物学领域，成倍地增强了我们对细胞、免疫学和微生物学的理解。流式细胞术最近催生了流式病毒测定法，这是一种检测、分析和表征单个病毒颗粒的新方法。由于当前流式细胞仪、校准和调谐方法的改进，流式细胞仪检测病毒成为可能。我们总结了流动病毒测定法的最近诞生和新用途，以及该工具为推进病毒学领域的逐步演变。我们还讨论了用于识别和分析病毒的各种流式病毒测定方法。我们简要总结了流式病毒测定的其他应用，包括：病毒检测、定量、种群鉴别和病毒颗粒的抗原特性。最后，