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Dopamine assisted PMOXA/PAA brushes for their switchable protein adsorption/desorption†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-18 00:00:00 , DOI: 10.1039/c7tb02209c Chao Pan 1, 2, 3, 4, 5 , Xiaoru Liu 1, 2, 3, 4, 5 , Kai Gong 1, 2, 3, 4, 5 , Fatima Mumtaz 1, 2, 3, 4, 5 , Yanmei Wang 1, 2, 3, 4, 5
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-18 00:00:00 , DOI: 10.1039/c7tb02209c Chao Pan 1, 2, 3, 4, 5 , Xiaoru Liu 1, 2, 3, 4, 5 , Kai Gong 1, 2, 3, 4, 5 , Fatima Mumtaz 1, 2, 3, 4, 5 , Yanmei Wang 1, 2, 3, 4, 5
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Protein adsorption on interfaces immersed in biological fluids is a prevalent and mostly irreversible phenomenon of great importance and is often uncontrolled. Poly(2-methyl-2-oxazoline) (PMOXA) is shown to possess excellent protein-resistant properties, whereas proteins can be adsorbed deeply inside the swollen brushes of poly(acrylic acid) (PAA). In this work, mixed brushes of PMOXA/PAA were prepared by sequentially grafting amine-terminated PMOXA (PMOXA-NH2) and thiol-terminated PAA (PAA-SH) onto poly(dopamine) (PDA)-coated substrates. The constituents of the mixed brushes can be adjusted by changing the grafting time of PMOXA-NH2 or PAA-SH to the PDA-coated surfaces. PAA chains with different molecular weights in the mixed brushes of PMOXA/PAA were used to investigate the effect of the length of the PAA chains on the surface wetting and protein adsorption on the prepared interfaces. The prepared brushes were rigorously characterized in terms of the surface chemical composition, thickness and hydrophilicity using X-ray photoelectron spectroscopy (XPS), ellipsometry and the static water contact angle (WCA) tests. Protein adsorption/desorption on the brushes were monitored using fluorescence microscopy as well as surface plasmon resonance (SPR) for in situ and real-time measurements. The results demonstrated that the WCA on mixed brushes could be reversibly tuned with the variation of pH and I, the amount of bovine serum albumin (BSA) adsorbed on mixed brushes could be well regulated by the grafting time and the molecular weight of PAA-SH, and the maximal amount (87%) of adsorbed BSA could then be desorbed upon changing pH and I. The highly switchable protein adsorption/desorption properties are therefore a nice combination of the protein-resistance of PMOXA and the salt and pH-sensitivity of PAA.
中文翻译：
多巴胺辅助的PMOXA / PAA刷具有可转换的蛋白质吸附/解吸功能†
蛋白质在浸没在生物流体中的界面上的吸附是非常普遍且普遍不可逆的重要现象，并且通常不受控制。聚（2-甲基-2-恶唑啉）（PMOXA）被证明具有出色的蛋白质抗性，而蛋白质可以被聚丙烯酸（PAA）溶胀的刷子深深地吸收。在这项工作中，通过将胺封端的PMOXA（PMOXA-NH 2）和硫醇封端的PAA（PAA-SH）依次接枝到聚（多巴胺）（PDA）涂覆的基材上，制备了PMOXA / PAA的混合刷。可以通过改变PMOXA-NH 2的接枝时间来调节混合刷的成分或PAA-SH到PDA涂层的表面。使用PMOXA / PAA混合刷中分子量不同的PAA链来研究PAA链的长度对所制备界面上的表面润湿和蛋白质吸附的影响。使用X射线光电子能谱（XPS），椭圆偏振法和静态水接触角（WCA）测试对制备的刷子进行了严格的表面化学成分，厚度和亲水性表征。使用荧光显微镜以及表面等离振子共振（SPR）监测刷子上的蛋白质吸附/解吸，以进行原位和实时测量。结果表明，混合刷上的WCA可以随着pH和I的变化而可逆地进行调节。通过接枝时间和PAA-SH的分子量可以很好地调节混合刷上牛血清白蛋白（BSA）的吸附量，并通过改变pH值和pH值使吸附量最大（87％）的BSA脱附。我。因此，高度可切换的蛋白质吸附/解吸性质是PMOXA的蛋白质抗性与PAA的盐和pH敏感性的良好组合。
更新日期：2017-12-18
中文翻译：
多巴胺辅助的PMOXA / PAA刷具有可转换的蛋白质吸附/解吸功能†
蛋白质在浸没在生物流体中的界面上的吸附是非常普遍且普遍不可逆的重要现象，并且通常不受控制。聚（2-甲基-2-恶唑啉）（PMOXA）被证明具有出色的蛋白质抗性，而蛋白质可以被聚丙烯酸（PAA）溶胀的刷子深深地吸收。在这项工作中，通过将胺封端的PMOXA（PMOXA-NH 2）和硫醇封端的PAA（PAA-SH）依次接枝到聚（多巴胺）（PDA）涂覆的基材上，制备了PMOXA / PAA的混合刷。可以通过改变PMOXA-NH 2的接枝时间来调节混合刷的成分或PAA-SH到PDA涂层的表面。使用PMOXA / PAA混合刷中分子量不同的PAA链来研究PAA链的长度对所制备界面上的表面润湿和蛋白质吸附的影响。使用X射线光电子能谱（XPS），椭圆偏振法和静态水接触角（WCA）测试对制备的刷子进行了严格的表面化学成分，厚度和亲水性表征。使用荧光显微镜以及表面等离振子共振（SPR）监测刷子上的蛋白质吸附/解吸，以进行原位和实时测量。结果表明，混合刷上的WCA可以随着pH和I的变化而可逆地进行调节。通过接枝时间和PAA-SH的分子量可以很好地调节混合刷上牛血清白蛋白（BSA）的吸附量，并通过改变pH值和pH值使吸附量最大（87％）的BSA脱附。我。因此，高度可切换的蛋白质吸附/解吸性质是PMOXA的蛋白质抗性与PAA的盐和pH敏感性的良好组合。
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