An ultraviolet (UV) light-triggered nanocarbon hybrid is developed for controlled C₆₀ release with excellent nitric oxide (NO) quenching ability. This nanocarrier, consisting of reduced graphene oxide (rGO) and β-cyclodextrin (β-CD), is capable of hosting azobenzene functionalized C₆₀ (Azo–C₆₀) synthesized by diazo chemistry. The hybridization of rGO, β-CD and Azo–C₆₀ enhances cellular uptake and limits the aggregation of C₆₀, and shows enhanced protective effects on NO-induced cytotoxicity. More interestingly, azo groups can reversibly switch between trans- and cis-isomers upon UV irradiation, so that the Azo–C₆₀ molecules exhibit photo-controlled release from rGO/β-CD in living cells. In vitro studies show that rGO/β-CD/C₆₀ treated with UV irradiation causes higher NO scavenging efficacy, which further significantly increases the cell viability from 32.6% to 88.4% at low loading levels (50 μg mL⁻¹). This represents an excellent NO quenching efficiency, better than other reports of the graphene/C₆₀ nanohybrids, and indicates that this material can be an effective nanoplatform to combat oxidative damage. As the host–guest chemistry and diazo chemistry are versatile and universally applicable, it is worth noting that the present strategy can also be applied in preparing other photo-responsive nanohybrids, which should be valuable for use in life science and materials science.

中文翻译：

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从石墨烯/环糊精纳米平台上光 触发的C ₆₀释放，用于保护一氧化氮诱导的细胞毒性†

开发了一种紫外线（UV）激发的纳米碳杂化物，用于控制C _60的释放，并具有出色的一氧化氮（NO）猝灭能力。这种由还原的氧化石墨烯（rGO）和β-环糊精（β-CD）组成的纳米载体能够容纳重氮化学合成的偶氮苯官能化的C ₆₀（Azo–C ₆₀）。rGO，β-CD和Azo–C ₆₀的杂交增强了细胞吸收并限制了C ₆₀的聚集，并显示出对NO诱导的细胞毒性的增强保护作用。更有趣的是，偶氮基团能够可逆地之间切换的反式-和顺-异构体在UV照射，使偶氮-C ₆₀分子在活细胞中显示出从rGO /β-CD的光控释放。体外研究表明，用UV辐射处理过的rGO /β-CD/ C ₆₀具有更高的NO清除效率，这在低负荷水平（50μgmL ^-1）下将细胞活力进一步从32.6％显着提高到88.4％。这代表了极好的NO猝灭效率，优于其他有关石墨烯/ C _60的报道纳米杂化物，并表明该材料可以是对抗氧化损伤的有效纳米平台。由于宿主-客体化学和重氮化学是通用的并且可以普遍应用，因此值得注意的是，本发明的策略也可以用于制备其他光响应性纳米杂化物，这对于生命科学和材料科学而言应该是有价值的。