Abstract Physical and chemical cues, which have emerged as a promising strategy for regulating cellular behaviors, provide important signaling cues to living cells. Neurons are also exposed to distinguishing physical and chemical environments that can greatly influence their behaviors and functions. In this study, we proposed the laminin-coated matrix nanotopography platforms (LMNPs) that generate extracellular physical and chemical cues for neuronal development. Using our platforms, we showed that nanotopographical and biochemical cues could provide suitable environments for neuronal cultures. More importantly, we showed that a LMNPs could control the orientation of neuronal structures as well as accelerate neuronal development through synergistic effects of extracellular nanotopographical and chemical cues. Our study imparts new design principles on the role of nanotopographical and chemical cues in neuronal development for the fabrication of neuroprosthetic scaffolds.

中文翻译：

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用于加速神经元发育的受控细胞外地形和化学线索

摘要 物理和化学线索已成为调节细胞行为的一种有前景的策略，为活细胞提供重要的信号线索。神经元还暴露于可极大影响其行为和功能的不同物理和化学环境中。在这项研究中，我们提出了层粘连蛋白涂层基质纳米拓扑平台（LMNPs），该平台为神经元发育产生细胞外物理和化学线索。使用我们的平台，我们表明纳米拓扑和生化线索可以为神经元培养提供合适的环境。更重要的是，我们表明LMNPs可以通过细胞外纳米拓扑学和化学线索的协同作用控制神经元结构的方向并加速神经元发育。