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Amperometric myeloperoxidase immunoassay based on the use of CuPdPt nanowire networks
Microchimica Acta ( IF 5.3 ) Pub Date : 2017-12-18 , DOI: 10.1007/s00604-017-2563-y
Yilin Wen , Jianyong Yuan , Jun Chen , Yilin Zhao , Yazhen Niu , Chao Yu

AbstractThis research describes a nanowire network-based method for detecting the activity of myeloperoxidase (MPO), a biomarker of acute coronary syndromes (ACS). Trimetallic CuPdPt nanowire networks (CuPdPt NWNWs) were prepared by a one-step chemical reduction method. The metallic precursors quickly form nanowire network structures without the need for additional capping agents or surfactants. This process creates a product with a clean surface. The NWNWs were dropped onto a glassy carbon electrode (GCE) to obtain a sensor with good catalytic activity towards the reduction of hydrogen peroxide (H2O2), which was used as an electrochemical probe working at −0.4 V (vs. SCE). It also provided a large surface for further modification. Next, an antibody against MPO was immobilized on the modified GCE via the stable conjunction between Cu, Pt, Pd and amino groups. Upon binding of MPO to the antibody on the GCE, the current response to H2O2 was reduced by 35 μA·cm−2. The immunosensor had a linear response within the 100 fg·mL−1 to 50 ng·mL−1 MPO concentration range and a 33 fg·mL−1 detection limit (at an S/N ratio of 3). The recovery of spiked serum samples ranged from 99.8 to 103.6%. This result suggests that the method can be applied to the quantitation of MPO in human serum samples. Graphical abstractA trimetallic CuPdPt nanowire networks was placed on a glassy cabon electrode (GCE) to design an immunosensor for myeloperoxidase (MPO), a biomarker for the acute coronary syndrome (ACS). Antibody against MPO was immobilized on the network via conjugation between Cu, Pt, Pd and amino groups. Amperometric i-t measurements were conducted to quantify the amount of MPO that binds to the antibody on the surface of the modified GCE.

中文翻译:

基于使用 CuPdPt 纳米线网络的电流法髓过氧化物酶免疫测定

摘要本研究描述了一种基于纳米线网络的方法,用于检测髓过氧化物酶 (MPO) 的活性,这是一种急性冠状动脉综合征 (ACS) 的生物标志物。三金属 CuPdPt 纳米线网络 (CuPdPt NWNWs) 通过一步化学还原方法制备。金属前体快速形成纳米线网络结构,无需额外的封端剂或表面活性剂。此过程创建具有清洁表面的产品。将 NWNW 滴在玻璃碳电极 (GCE) 上以获得对过氧化氢 (H2O2) 的还原具有良好催化活性的传感器,该传感器用作在 -0.4 V (vs. SCE) 下工作的电化学探针。它还为进一步修改提供了一个大的表面。接下来,通过 Cu、Pt、Pd 和氨基。MPO 与 GCE 上的抗体结合后,对 H2O2 的电流响应降低了 35 μA·cm-2。免疫传感器在 100 fg·mL-1 至 50 ng·mL-1 MPO 浓度范围内具有线性响应,检测限为 33 fg·mL-1(信噪比为 3)。加标血清样品的回收率为 99.8% 至 103.6%。该结果表明该方法可用于定量人血清样品中的 MPO。图形摘要将三金属 CuPdPt 纳米线网络放置在玻璃碳电极 (GCE) 上以设计髓过氧化物酶 (MPO) 的免疫传感器,髓过氧化物酶 (MPO) 是急性冠状动脉综合征 (ACS) 的生物标志物。针对 MPO 的抗体通过 Cu、Pt、Pd 和氨基之间的结合固定在网络上。
更新日期:2017-12-18
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