A film consisting of poly(γ-glutamic acid) modified with 3-aminothiophene (ATh-γ-PGA) was prepared by macromolecular self-assembly and electropolymerization. ATh-γ-PGA is amphiphilic and electrically conductive. The copolymers undergo self-assembly to form nanoparticles (NPs) on decreasing the pH value of an aqueous solution. A conducting film of NPs was formed on the surface of a gold electrode by casting the ATh-γ-PGA NPs and subsequently electropolymerizing the thiophene units. Next, horseradish peroxidase and Nafion were cast onto the film to obtain an enzymatic biosensor for H2O2. Due to the electropolymerization step, a cross-conjugated polymer network is created that improves electron transfer rates and thus enhances the response. This endows the biosensor with high sensitivity. Two linear ranges are present, the first ranging from 1 × 10−11 to 1 × 10−8 mol·L−1, and the second from 1 × 10−8 to 1 × 10−5 mol·L−1. The detection limit is as low as 3 × 10−12 mol·L−1. The sensor is stable, repeatable, and was successfully applied to the determination of H2O2 in a commercial disinfecting solution.Graphical abstractPreparation of a conducting nanoparticle (NP) film on the gold electrode (GE) by self-assembly of poly(γ-glutamic acid) that was modified with electroactive 3-aminothiophene (ATh-γ-PGA). It served as a platform for the fabricationof an ultrasensitive voltammetric enzyme-based biosensor for H2O2.

中文翻译：

---

用由导电共聚物组成的纳米颗粒膜修饰的金电极，用于过氧化氢的超灵敏伏安传感

通过大分子自组装和电聚合制备了由 3-氨基噻吩（ATh-γ-PGA）改性的聚（γ-谷氨酸）组成的薄膜。ATh-γ-PGA 是两亲性和导电性的。共聚物在降低水溶液的 pH 值时进行自组装以形成纳米颗粒 (NPs)。通过浇铸 ATh-γ-PGA NPs 并随后对噻吩单元进行电聚合，在金电极表面形成了 NPs 导电膜。接下来，将辣根过氧化物酶和 Nafion 浇铸到薄膜上以获得 H2O2 的酶促生物传感器。由于电聚合步骤，产生了交叉共轭聚合物网络，可提高电子转移速率，从而增强响应。这赋予生物传感器高灵敏度。存在两个线性范围，第一个范围从 1 × 10-11 到 1 × 10-8 mol·L-1，第二个范围从 1 × 10-8 到 1 × 10-5 mol·L-1。检测限低至 3 × 10-12 mol·L-1。该传感器稳定、可重复，并成功应用于测定商用消毒溶液中的 H2O2。 ) 用电活性 3-氨基噻吩 (ATh-γ-PGA) 修饰。它用作制造超灵敏伏安法基于酶的 H2O2 生物传感器的平台。图形摘要通过用电活性 3-氨基噻吩 (ATh-γ-PGA) 修饰的聚 (γ-谷氨酸) 自组装在金电极 (GE) 上制备导电纳米颗粒 (NP) 膜。它用作制造超灵敏伏安法基于酶的 H2O2 生物传感器的平台。图形摘要通过用电活性 3-氨基噻吩 (ATh-γ-PGA) 修饰的聚 (γ-谷氨酸) 自组装在金电极 (GE) 上制备导电纳米颗粒 (NP) 膜。它用作制造超灵敏伏安法基于酶的 H2O2 生物传感器的平台。