Sustained Delivery of BMP-2-Related Peptide from the True Bone Ceramics/Hollow Mesoporous Silica Nanoparticles Scaffold for Bone Tissue Regeneration,ACS Biomaterials Science & Engineering

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Sustained Delivery of BMP-2-Related Peptide from the True Bone Ceramics/Hollow Mesoporous Silica Nanoparticles Scaffold for Bone Tissue Regeneration
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-12-26 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00506
Wei Cui _{1,

2} , Qianqian Liu ₃ , Liang Yang ₁ , Ke Wang ₃ , Tingfang Sun ₁ , Yanhui Ji ₁ , Liping Liu ₃ , Wei Yu ₁ , Yanzhen Qu ₁ , Junwen Wang ₂ , Zhigang Zhao ₂ , Jintao Zhu ₃ , Xiaodong Guo ₁

Affiliation

Bone morphogenetic protein 2 (BMP-2) is one of the most important factors for bone tissue formation. A number of BMP-2 related small molecule bioactive peptides have been designed and shown to be equally effective in osteogenic activity. In this report, we synthesized a novel BMP-2-related peptide (designated P28) and designed a delivery system to regulate the controlled release of P28 from true bone ceramics (TBC) combined with an enlarged pore hollow mesoporous silica nanoparticles (HMSNs) composite scaffold. An in vitro release showed that the release of P28 from the TBC/HMSN scaffold was slower than that from the TBC scaffold. An in vitro cell experiment of the TBC/HMSN/P28 scaffold was tested with MC3T3-E1 cells in comparison to TBC, TBC/HMSN, and TBC/P28 scaffolds. Our results demonstrated that the TBC/HMSN/P28 scaffold had better effects on promoting proliferation and osteogenic differentiation of MC3T3-E1 cells than TBC, TBC/HMSN, and TBC/P28 scaffolds. After four kinds of scaffolds were implanted into a rabbit radius critical bone defect for 6 and 12 weeks, the radiographic and histological examination indicated that this osteogenic delivery system TBC/HMSN/P28 scaffold effectively induced bone regeneration in vivo. Therefore, the TBC/HMSN/P28 scaffold can promote proliferation and osteogenic differentiation of MC3T3-E1 cells in vitro and new bone tissue generation in vivo. This study provides a promising scaffold for bone tissue engineering and regenerative medicine.

中文翻译：

从用于骨组织再生的真骨陶瓷/空心介孔二氧化硅纳米颗粒支架持续递送 BMP-2 相关肽

骨形态发生蛋白 2 (BMP-2) 是骨组织形成的最重要因素之一。许多 BMP-2 相关的小分子生物活性肽已被设计并显示在成骨活性中同样有效。在本报告中，我们合成了一种新型 BMP-2 相关肽（命名为 P28），并设计了一种递送系统来调节 P28 从真骨陶瓷 (TBC) 中的受控释放，结合扩大孔隙中空介孔二氧化硅纳米粒子 (HMSN) 复合材料脚手架。体外释放表明，P28 从 TBC/HMSN 支架的释放比从 TBC 支架的释放慢。体外_与 TBC、TBC/HMSN 和 TBC/P28 支架相比，使用 MC3T3-E1 细胞测试了 TBC/HMSN/P28 支架的细胞实验。我们的研究结果表明，TBC/HMSN/P28 支架比 TBC、TBC/HMSN 和 TBC/P28 支架对促进 MC3T3-E1 细胞的增殖和成骨分化具有更好的作用。将四种支架植入兔桡骨严重骨缺损处 6 周和 12 周后，放射学和组织学检查表明，这种成骨输送系统 TBC/HMSN/P28 支架有效地诱导了体内骨再生。因此，TBC/HMSN/P28支架可以促进MC3T3-E1细胞体外增殖和成骨分化，以及体内新骨组织的生成. 该研究为骨组织工程和再生医学提供了一个有前途的支架。

更新日期：2017-12-26

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