Testing the limits of modification: Nucleosides containing adducts at the O⁴ atom of 5‐fluoro‐2′‐deoxyuridine have been synthesised and incorporated into oligonucleotides by solid‐phase synthesis. The C5‐fluorine‐modified nucleosides display enhanced susceptibility towards removal of the O⁴‐alkyl group by O⁶‐alkylguanine DNA alkyltransferases (AGTs).

中文翻译：

---

O6-烷基鸟嘌呤DNA烷基转移酶对O4-烷基化-2-脱氧尿苷的修复作用是通过C5-氟修饰修饰来增强的

测试修饰的极限：已经合成了在5-氟-2'-脱氧尿苷的O ⁴原子处包含加合物的核苷，并通过固相合成将其掺入寡核苷酸中。C5-氟修饰的核苷显示出对O ^6-烷基鸟嘌呤DNA烷基转移酶（AGT）去除O ^4-烷基的敏感性更高。