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Perfluoroalkyl Substances Increase the Membrane Permeability and Quorum Sensing Response in Aliivibrio fischeri
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2017-12-15 00:00:00 , DOI: 10.1021/acs.estlett.7b00518 Nicole J. M. Fitzgerald 1 , Matt F. Simcik 2 , Paige J. Novak 1
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2017-12-15 00:00:00 , DOI: 10.1021/acs.estlett.7b00518 Nicole J. M. Fitzgerald 1 , Matt F. Simcik 2 , Paige J. Novak 1
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Perfluoroalkyl substances (PFAS) are used in a variety of products and are ubiquitous in the environment. They have been found to associate with eukaryotic cell membranes and alter membrane properties. Bacteria are exposed to elevated concentrations of PFAS in some environments; nevertheless, the effect of PFAS exposure on microbial membranes has not yet been studied. Some quorum sensing pathways require the passive diffusion of signaling molecules through cell membranes. Quorum sensing initiates a variety of bacterial processes, such as biofilm formation and antibiotic production. If PFAS exposure increased the microbial quorum sensing response, these processes could be initiated at lower population densities, with wide-ranging ramifications for PFAS-impacted environments. This study examined the effect of perfluorinated alkyl sulfonates and carboxylates on quorum sensing in a model bacterium, Aliivibrio fischeri. Results showed that cultures exposed to PFAS were brighter after they received the signaling molecule. The observed increase in luminescence was dose-dependent and increased with the fluorinated carbon number. Specifically, perfluorooctanesulfonate increased luminescence at levels as low as 10 μg/L. PFAS-exposed bacteria were also more permeable to a semi-membrane permeable dye. Therefore, it is likely that increased permeability was, at least in part, the cause of increased luminescence.
中文翻译：
全氟烷基物质增加了拟南芥的膜通透性和群体感应反应
全氟烷基物质（PFAS）用于多种产品，并且在环境中无处不在。已经发现它们与真核细胞膜缔合并改变膜性质。在某些环境中，细菌会暴露于高浓度的PFAS中。但是，尚未研究PFAS暴露对微生物膜的影响。一些群体感应途径需要信号分子通过细胞膜的被动扩散。群体感应可引发多种细菌过程，例如生物膜形成和抗生素生产。如果PFAS暴露增加了微生物群体感测响应，则可以在较低的人口密度下启动这些过程，并对受PFAS影响的环境产生广泛的影响。拟南芥（Aliivibrio fischeri）。结果表明，暴露于PFAS的培养物在收到信号分子后变亮。观察到的发光增加是剂量依赖性的，并且随氟化碳数的增加而增加。具体而言，全氟辛烷磺酸盐的发光强度可低至10μg/ L。暴露于PFAS的细菌对半膜可渗透染料的渗透性也更高。因此，增加的渗透性可能至少部分是发光增加的原因。
更新日期：2017-12-15
中文翻译：
全氟烷基物质增加了拟南芥的膜通透性和群体感应反应
全氟烷基物质（PFAS）用于多种产品，并且在环境中无处不在。已经发现它们与真核细胞膜缔合并改变膜性质。在某些环境中，细菌会暴露于高浓度的PFAS中。但是，尚未研究PFAS暴露对微生物膜的影响。一些群体感应途径需要信号分子通过细胞膜的被动扩散。群体感应可引发多种细菌过程，例如生物膜形成和抗生素生产。如果PFAS暴露增加了微生物群体感测响应，则可以在较低的人口密度下启动这些过程，并对受PFAS影响的环境产生广泛的影响。拟南芥（Aliivibrio fischeri）。结果表明，暴露于PFAS的培养物在收到信号分子后变亮。观察到的发光增加是剂量依赖性的，并且随氟化碳数的增加而增加。具体而言，全氟辛烷磺酸盐的发光强度可低至10μg/ L。暴露于PFAS的细菌对半膜可渗透染料的渗透性也更高。因此，增加的渗透性可能至少部分是发光增加的原因。
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