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Improved cell adhesion and osteogenesis using a PLTGA (poly l-lactide, 1,3-trimethylene carbonate, and glycolide) terpolymer by gelatin-assisted hydroxyapatite immobilization for bone regeneration
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-14 00:00:00 , DOI: 10.1039/c7tb02293j Qian Chen 1, 2, 3, 4, 5 , Lu Cao 1, 2, 3, 4, 5 , Jie-Lin Wang 3, 5, 6, 7 , Hang Zhao 3, 5, 6, 7 , Hong Lin 1, 2, 3, 4, 5 , Zhong-Yong Fan 3, 5, 6, 7 , Jian Dong 1, 2, 3, 4, 5
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-14 00:00:00 , DOI: 10.1039/c7tb02293j Qian Chen 1, 2, 3, 4, 5 , Lu Cao 1, 2, 3, 4, 5 , Jie-Lin Wang 3, 5, 6, 7 , Hang Zhao 3, 5, 6, 7 , Hong Lin 1, 2, 3, 4, 5 , Zhong-Yong Fan 3, 5, 6, 7 , Jian Dong 1, 2, 3, 4, 5
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A PLTGA (poly L-lactide, trimethylene carbonate, glycolide) terpolymer possesses great potential for orthopedic applications due to its excellent biocompatibility and controllable biodegradability. However, the unfavorable surface conditions of bare PLTGA such as its poor hydrophilicity and smooth morphology impede its clinical applications, highlighting the need for tailored surface modifications to improve its cytological behavior and osteogenic capacity. We herein develop a facile and effective strategy to deposit a gelatin/hydroxyapatite (GEL/HAP) hybrid coating onto the surface of PLTGA that involves consecutive chemical grafting and in situ reaction steps. Following the surface modification treatment, the resultant PLTGA scaffold with the GEL/HAP coating exhibited drastically improved hydrophilicity (79.1° vs. 48.2° in water contact angle) and increased surface roughness (18.4 vs. 267.9 nm, more than 14-fold, in root-mean-square roughness), respectively. In addition, preosteoblast (MC3T3-E1) cells were seeded onto the bare/modified PLTGA scaffold to evaluate biological performance, including cell adhesion, proliferation, mineralization and osteogenic differentiation. Based on these results, the GEL/HAP hybrid coating can endow the PLTGA terpolymer substrate with enhanced cell adhesion, proliferation and osteogenic functionality. Overall, post-treatment of PLTGA with the GEL/HAP hybrid coating may be a promising methodology in bone regeneration applications.
中文翻译：
使用明胶辅助羟基磷灰石固定化PLTGA（聚l-丙交酯，1,3-三亚甲基碳酸酯和乙交酯）三元共聚物改善细胞粘附和成骨作用
PLTGA（聚L-丙交酯，碳酸亚丙酯，乙交酯）三元共聚物具有出色的生物相容性和可控的生物降解性，因此在骨科领域具有巨大的潜力。但是，裸露的PLTGA的不利表面条件（如亲水性差和光滑的形态）阻碍了其临床应用，突出显示了需要进行定制的表面修饰以改善其细胞学行为和成骨能力。我们在本文中开发了一种简便有效的策略，可将明胶/羟基磷灰石（GEL / HAP）杂化涂层沉积到PLTGA的表面上，该涂层涉及连续的化学接枝和原位反应步骤。经过表面改性处理后，所得的具有GEL / HAP涂层的PLTGA支架的亲水性显着提高（水接触角为79.1°对48.2°），表面粗糙度提高了（18.4对表面。均方根粗糙度为267.9 nm，大于14倍）。此外，将成骨细胞（MC3T3-E1）细胞接种到裸露的/修饰的PLTGA支架上，以评估生物学性能，包括细胞粘附，增殖，矿化和成骨分化。基于这些结果，GEL / HAP杂化涂层可以赋予PLTGA三元共聚物底物增强的细胞粘附性，增殖能力和成骨功能。总体而言，在骨再生应用中，用GEL / HAP混合涂层对PLTGA进行后处理可能是一种很有前途的方法。
更新日期：2017-12-14
中文翻译：
使用明胶辅助羟基磷灰石固定化PLTGA（聚l-丙交酯，1,3-三亚甲基碳酸酯和乙交酯）三元共聚物改善细胞粘附和成骨作用
PLTGA（聚L-丙交酯，碳酸亚丙酯，乙交酯）三元共聚物具有出色的生物相容性和可控的生物降解性，因此在骨科领域具有巨大的潜力。但是，裸露的PLTGA的不利表面条件（如亲水性差和光滑的形态）阻碍了其临床应用，突出显示了需要进行定制的表面修饰以改善其细胞学行为和成骨能力。我们在本文中开发了一种简便有效的策略，可将明胶/羟基磷灰石（GEL / HAP）杂化涂层沉积到PLTGA的表面上，该涂层涉及连续的化学接枝和原位反应步骤。经过表面改性处理后，所得的具有GEL / HAP涂层的PLTGA支架的亲水性显着提高（水接触角为79.1°对48.2°），表面粗糙度提高了（18.4对表面。均方根粗糙度为267.9 nm，大于14倍）。此外，将成骨细胞（MC3T3-E1）细胞接种到裸露的/修饰的PLTGA支架上，以评估生物学性能，包括细胞粘附，增殖，矿化和成骨分化。基于这些结果，GEL / HAP杂化涂层可以赋予PLTGA三元共聚物底物增强的细胞粘附性，增殖能力和成骨功能。总体而言，在骨再生应用中，用GEL / HAP混合涂层对PLTGA进行后处理可能是一种很有前途的方法。
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