In vivo tumor active cancer targeting and CT-fluorescence dual-modal imaging with nanoprobe based on gold nanorods and InP/ZnS quantum dots†,Journal of Materials Chemistry B

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In vivo tumor active cancer targeting and CT-fluorescence dual-modal imaging with nanoprobe based on gold nanorods and InP/ZnS quantum dots†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-12-14 00:00:00 , DOI: 10.1039/c7tb02643a
Lin Zhang _{1,

2,

3,

4,

5} , Xiao-Quan Yang _{1,

2,

3,

4,

5} , Jie An _{1,

2,

3,

4,

5} , Sun-Duo Zhao _{1,

2,

3,

4,

5} , Tian-Yu Zhao _{1,

2,

3,

4,

5} , Fang Tan _{6,

7,

8,

9,

10} , Yuan-Cheng Cao _{6,

7,

8,

9,

10} , Yuan-Di Zhao _{1,

2,

3,

4,

5}

Affiliation

In this paper, gold nanorods and InP/ZnS quantum dots were encapsulated together in a silica medium, and the targeting molecular peptide c(RGDfC) was further connected after surface modification with PEG and PEG derivatives to prepare a multifunctional Au@QD@SiO₂/PEG-c(RGDfC) probe. Dynamic Light Scattering showed that the probe size was about 215.01 ± 2.72 nm, and its dispersibility was good. In in vitro experiments when the concentration was as high as 200 μg mL⁻¹, the activity of the cells was still 85% due to low toxicity. In vivo experiments showed that the probe had excellent tumor targeting, X-ray computed tomography (CT) imaging and fluorescence imaging capabilities. The experiments revealed that the probe had a long blood circulation time (T_1/2 = 7.78 h) in mice. Biochemical analysis, liver enzyme analysis and histomorphological analysis after probe injection showed that the probe had no obvious side effects on the normal functions of the main organs, indicating good biosafety. In vivo imaging experiments showed that 6 d after intravenous injection, the tumor sites of a HeLa tumor-bearing nude mice positive group presented obvious fluorescence and CT signals, indicating that the prepared nanoprobe had good tumor targeting dual-mode imaging capabilities and therefore showed great potential in biomedical imaging applications, especially the diagnosis of cancer.

中文翻译：

基于金纳米棒和InP / ZnS量子点的纳米探针的体内肿瘤活性癌症靶向和CT荧光双峰成像†

本文将金纳米棒和InP / ZnS量子点一起包裹在二氧化硅介质中，并用PEG和PEG衍生物表面修饰后进一步连接目标分子肽c（RGDfC），以制备多功能Au @ QD @ SiO ₂ / PEG-c（RGDfC）探针。动态光散射显示探针尺寸约为215.01±2.72nm，并且其分散性良好。在体外实验中，当浓度高达200μgmL ^-1时，由于低毒性，细胞的活性仍为85％。体内实验表明，该探针具有出色的肿瘤靶向，X射线计算机断层扫描（CT）成像和荧光成像功能。实验表明，该探针在小鼠中的血液循环时间较长（T _1/2 = 7.78 h）。探针注射后的生化分析，肝酶分析和组织形态学分析表明，该探针对主要器官的正常功能没有明显的副作用，表明具有良好的生物安全性。体内成像实验表明，静注HeLa的荷瘤裸鼠阳性组在注射后6 d，肿瘤部位具有明显的荧光和CT信号，表明所制备的纳米探针具有良好的靶向肿瘤的双模成像能力，因此具有很大的发展潜力。生物医学成像应用，尤其是癌症的诊断。

更新日期：2017-12-14

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