Surface-enhanced Raman spectroscopy (SERS) is a promising and emerging technique to analyze the cellular environment. We developed an alternative, rapid and label-free SERS-based method to get information about the cellular environment by analyzing cells lysates, thus avoiding the need to incorporate nanoparticles into cells. Upon sonicating and filtrating cells, we obtained lysates which, mixed with Au or Ag nanoparticles, yield stable and repeatable SERS spectra, whose overall profile depends on the metal used as substrate, but not on the buffer used for the lysis process. Bands appearing in these spectra were shown to arise mostly from the cytosol and were assigned to adenine, guanine, adenosine and reduced glutathione (GSH). Spectral differences among various cell types also demonstrated that this approach is suitable for cell type identification.

中文翻译：

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细胞的 SERS：我们可以从细胞裂解物中学到什么？

表面增强拉曼光谱 (SERS) 是一种很有前途的新兴技术，用于分析细胞环境。我们开发了一种替代的、快速的、无标记的基于 SERS 的方法，通过分析细胞裂解物来获取有关细胞环境的信息，从而避免将纳米颗粒掺入细胞中。在对细胞进行超声处理和过滤后，我们获得了与 Au 或 Ag 纳米粒子混合的裂解物，可产生稳定且可重复的 SERS 光谱，其总体特征取决于用作底物的金属，而不取决于用于裂解过程的缓冲液。出现在这些光谱中的条带主要来自细胞质，并被指定为腺嘌呤、鸟嘌呤、腺苷和还原型谷胱甘肽 (GSH)。