In the present study, 155 Nellore cattle were genotyped for the CAPN4751 and UOGCAST polymorphisms and phenotyped for shear force (SF) at 1, 7 and 14 days aging. The effects of different genotypic combinations were evaluated on the Longissimus muscle proteomic profile using 2DE and mass spectrometry. A significant association was found between genotypes for UOGCAST and CAPN4751 and meat tenderness. The CC genotype for both markers was favorable for lesser shear force than TT. A total of 40 spots showed significant differential expression profiles (P < 0.05), of which eight had a main effect for the CAPN4751 marker, 11 for UOGCAST, two for both markers, and 19 had interactions between markers, including myosin (MYL1, MYL2, MYLPF and MYL6B), actin (ACTA1 and CAPZβ), troponin (TNNT1 and TNNT3) and heat shock proteins (HSPB6, HSPB1 and HSP70-2). The results demonstrated that UOGCAST and CAPN4751 genotypes led to variability on the expression of proteins that are involved in muscle metabolism, and consequently affect meat tenderness.

在本研究中，对155头Nellore牛进行了CAPN4751和UOGCAST多态性的基因分型，并针对了在第1、7和14天时的剪切力（SF）进行了表型化。在Longissimus上评估了不同基因型组合的影响使用2DE和质谱分析肌肉蛋白质组学特征。发现UOGCAST和CAPN4751的基因型与肉嫩度之间存在显着关联。与TT相比，两种标记物的CC基因型均具有较小的剪切力。共有40个斑点显示出显着的差异表达谱（P <0.05），其中8个对CAPN4751标记有主要作用，11个对UOGCAST有影响，两个对两个标记都有影响，19个在标记之间有相互作用，包括肌球蛋白（MYL1，MYL2 ，MYLPF和MYL6B），肌动蛋白（ACTA1和CAPZβ），肌钙蛋白（TNNT1和TNNT3）和热激蛋白（HSPB6，HSPB1和HSP70-2）。结果表明，UOGCAST和CAPN4751基因型导致参与肌肉代谢的蛋白质表达变化，从而影响肉的嫩度。