High concentrations of aldehyde-based cross-linkers have been commonly used for protein immobilization to facilitate microscale and nanoscale observations. This fixation maintains cell morphology and partial protein activity. In this study, a facile one-step strategy based on a similar concept was first developed for the bioprosthesis of a uniform core–shell gold nanorod/serum albumin ([email protected]) nanoplatform. The resultant albumin shell preserved half of its native form, leading to decreased free SA adsorption, and even these adsorbed proteins were close to their native form. This strategy efficiently prevents subsequent adsorption cascades of other proteins and has a remarkable influence on cellular uptake (of macrophages and tumor cells). Furthermore, the other, artificial part endowed [email protected] with higher drug loading capacity and enhanced photoacoustic signal intensity for cancer theranostics compared with those of its pristine counterpart. These findings suggested that preserved fidelity and artificial characterizations provide a new perspective for biomimetic nanomaterial design.

中文翻译：

---

核壳金纳米棒/血清白蛋白纳米仿制的生物假体：癌症治疗学的半天然和半人工纳米杂交。

高浓度的基于醛的交联剂已普遍用于蛋白质固定化，以促进微观和纳米尺度的观察。这种固定保持细胞形态和部分蛋白质活性。在这项研究中，首先开发了一种基于相似概念的简便的单步策略，用于生物义肢形成均匀的核壳金纳米棒/血清白蛋白（[受电子邮件保护]）纳米平台。所得的白蛋白壳保留了其天然形式的一半，导致游离SA吸附减少，甚至这些吸附的蛋白质也接近其天然形式。该策略有效地防止了其他蛋白质的后续吸附级联，并且对细胞摄取（巨噬细胞和肿瘤细胞）具有显着影响。此外，其他 与原始部分相比，人工部分具有更高的载药量和增强的光声信号强度，可用于癌症治疗学。这些发现表明，保留的保真度和人工表征为仿生纳米材料设计提供了新的视角。