The Future of Multiplexed Eukaryotic Genome Engineering,ACS Chemical Biology

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The Future of Multiplexed Eukaryotic Genome Engineering
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2017-12-28 00:00:00 , DOI: 10.1021/acschembio.7b00842
David B Thompson _{1,

2} , Soufiane Aboulhouda _{1,

2} , Eriona Hysolli _{1,

2} , Cory J Smith _{1,

2} , Stan Wang _{1,

2} , Oscar Castanon _{1,

2,

3} , George M Church _{1,

2}

Affiliation

Multiplex genome editing is the simultaneous introduction of multiple distinct modifications to a given genome. Though in its infancy, maturation of this field will facilitate powerful new biomedical research approaches and will enable a host of far-reaching biological engineering applications, including new therapeutic modalities and industrial applications, as well as “genome writing” and de-extinction efforts. In this Perspective, we focus on multiplex editing of large eukaryotic genomes. We describe the current state of multiplexed genome editing, the current limits of our ability to multiplex edits, and provide perspective on the many applications that fully realized multiplex editing technologies would enable in higher eukaryotic genomes. We offer a broad look at future directions, covering emergent CRISPR-based technologies, advances in intracellular delivery, and new DNA assembly approaches that may enable future genome editing on a massively multiplexed scale.

中文翻译：

多重真核基因组工程的未来

多重基因组编辑是对给定基因组同时引入多种不同的修饰。尽管该领域还处于起步阶段，但其成熟将促进强大的新生物医学研究方法的发展，并将实现一系列影响深远的生物工程应用，包括新的治疗方式和工业应用，以及“基因组书写”和反灭绝努力。在这个视角中，我们专注于大型真核基因组的多重编辑。我们描述了多重基因组编辑的现状、多重编辑能力的当前限制，并提供了对完全实现多重编辑技术在高等真核基因组中实现的许多应用的看法。我们对未来的发展方向进行了广泛的展望，涵盖基于 CRISPR 的新兴技术、细胞内递送的进步以及可能使未来大规模多重规模的基因组编辑成为可能的新 DNA 组装方法。

更新日期：2017-12-28

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