A microscopic image of a section of a peanut seed shows that oil bodies (OBs) are a small droplet of oil that is dispersed throughout the whole seed. The protein profile of peanut's OBs recovered using the aqueous extraction method at different pH was found to have 2 oleosin isoforms of 14 and 16 kDa. Moreover, OBs essential amino acids are 1.52 higher than those in the PPI. Oleic acid and linoleic acid are the major fatty acids in both cold press peanut oil and OBs regardless of pH. Tocopherol content went from 270.76 to 278.2 mg/g when pH got increased. δ-Tocopherols are slightly associated with peanut OBs, as it was resistant to the alkaline washing; however, α-tocopherols were discovered to be weakly associable. On the contrary, phytosterols content decreased when pH got increased, with 631.49 μg/g for pH 6.8 and 614.96 μg/g for pH 11.0. PRACTICAL APPLICATION Peanut oil is widely used in food industries in the form of emulsified oil. OBs can be presented as a better alternative solution, which is stable emulsified oil, with high content of antioxidant and phytosterols. Our results showed that peanut OBs are rich in nutritional components such as essential amino acids, unsaturated fatty acids, vitamin E, and phytosterols. Moreover, peanut OBs are surrounded with 2 oleosin isoforms and caleosin, which offer more stability to the emulsion system. Thus, peanut OBs can be perfect for food like mayonnaise and vinaigrettes products in the form of encapsulated oil with high content of vitamin E.

中文翻译：

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花生油体整合蛋白、脂质及其相关植物化学物质的表征

花生种子切片的显微图像显示油体 (OB) 是分散在整个种子中的一小滴油。使用水提法在不同 pH 下回收的花生 OBs 的蛋白质谱被发现具有 14 和 16 kDa 的 2 种油质蛋白同工型。此外，OBs必需氨基酸比PPI高1.52。无论 pH 值如何，油酸和亚油酸都是冷榨花生油和 OB 中的主要脂肪酸。当 pH 值增加时，生育酚含量从 270.76 变为 278.2 mg/g。δ-生育酚与花生 OBs 略有关联，因为它耐碱洗；然而，发现α-生育酚是弱缔合的。相反，随着 pH 值的增加，植物甾醇含量下降，pH 6.8 为 631.49 μg/g，pH 11.0 为 614.96 μg/g。实际应用花生油以乳化油的形式广泛用于食品工业。OBs可以作为一种更好的替代解决方案，它是一种稳定的乳化油，具有高含量的抗氧化剂和植物甾醇。我们的研究结果表明，花生OBs富含必需氨基酸、不饱和脂肪酸、维生素E和植物甾醇等营养成分。此外，花生 OBs 周围有 2 种油质蛋白异构体和 caleosin，它们为乳液系统提供了更高的稳定性。因此，花生 OB 可以完美地制成具有高维生素 E 含量的封装油形式的蛋黄酱和香醋产品等食品。具有高含量的抗氧化剂和植物甾醇。我们的研究结果表明，花生OBs富含必需氨基酸、不饱和脂肪酸、维生素E和植物甾醇等营养成分。此外，花生 OBs 周围有 2 种油质蛋白异构体和 caleosin，它们为乳液系统提供了更高的稳定性。因此，花生 OB 可以完美地制成具有高维生素 E 含量的封装油形式的蛋黄酱和香醋产品等食品。具有高含量的抗氧化剂和植物甾醇。我们的研究结果表明，花生OBs富含必需氨基酸、不饱和脂肪酸、维生素E和植物甾醇等营养成分。此外，花生 OBs 周围有 2 种油质蛋白异构体和 caleosin，它们为乳液系统提供了更高的稳定性。因此，花生 OB 可以完美地制成具有高维生素 E 含量的封装油形式的蛋黄酱和香醋产品等食品。