In this paper, pectin-reinforced, supported liquid membranes (SLMs) prepared with carbonic anhydrase (CA) were investigated for CO₂/N₂ separation. In the first part of the study, the effect of [Bmim][NTf₂] ionic liquid (IL) – as possible solvent to fill the pores of cellulose acetate support during SLM fabrication – on enzyme activity was tested. It turned out that this particular IL caused rapid and severe loss of initial biocatalyst activity, which fact can be seen as a threat in the membrane process design. Afterwards, the stability of pectin-containing SLMs (containing CA but lacking the IL having adverse impact) was addressed and their improved resistance against higher transmembrane pressures (up to 7.2 bar) was found, representing an approx. 3-fold enhancement compared to their control. Thereafter, the performance of the membranes was tested under single and mixed gas conditions with carbon dioxide and nitrogen. Employing single gases, it was demonstrated that CA enzyme could notably increase CO₂ permeability (from 55 to 93 Barrer), while that of N₂ remained unchanged (1.6-1.7 Barrer). Thus, the highest CO₂/N₂ theoretical selectivity was attained as 54 using the pectin-reinforced SLMs enriched with CA biocatalyst. For comparison, the outcomes were plotted on the Robeson upper-bound.

本文研究了用碳酸酐酶（CA）制备的果胶增强的负载型液膜（SLM）的CO ₂ / N ₂分离。在研究的第一部分中，[Bmim] [NTf ₂测试了离子液体（IL）–在SLM制备过程中可能填充乙酸纤维素载体孔的溶剂–对酶的活性进行了测试。事实证明，这种特定的IL导致初始生物催化剂活性的快速和严重丧失，这一事实可被视为对膜工艺设计的威胁。此后，解决了含有果胶的SLM（含有CA但缺乏IL产生不利影响）的稳定性，发现它们对更高的跨膜压力（最高7.2 bar）具有更高的抵抗力，大约为。与对照相比，增强了3倍。此后，在单一和混合气体条件下用二氧化碳和氮气测试膜的性能。通过使用单一气体，证明了CA酶可以显着增加CO _{2的表达。}磁导率（从55到93 Barrer），而N _2的磁导率保持不变（1.6-1.7 Barrer）。因此，使用富含CA生物催化剂的果胶增强的SLM，获得了最高的CO ₂ / N ₂理论选择性，为54。为了进行比较，将结果绘制在Robeson上限上。