A novel and sensitive assay for aflatoxin B1 (AFB1) detection has been developed by using bio-bar code assay (BCA). The method that relies on polyclonal antibodies encoded with DNA modified gold nanoparticle (NP) and monoclonal antibodies modified magnetic microparticle (MMP), and subsequent detection of amplified target in the form of bio-bar code using a fluorescent quantitative polymerase chain reaction (FQ-PCR) detection method. First, NP probes encoded with DNA that was unique to AFB1, MMP probes with monoclonal antibodies that bind AFB1 specifically were prepared. Then, the MMP-AFB1-NP sandwich compounds were acquired, dehybridization of the oligonucleotides on the nanoparticle surface allows the determination of the presence of AFB1 by identifying the oligonucleotide sequence released from the NP through FQ-PCR detection. The bio-bar code techniques system for detecting AFB1 was established, and the sensitivity limit was about 10-8 ng/mL, comparable ELISA assays for detecting the same target, it showed that we can detect AFB1 at low attomolar levels with the bio-bar-code amplification approach. This is also the first demonstration of a bio-bar code type assay for the detection of AFB1 in Chinese herbs.

中文翻译：

---

基于纳米颗粒的生物条形码技术对中草药中黄曲霉毒素B1的痕量分析

通过使用生物条形码检测 (BCA) 开发了一种新的、灵敏的黄曲霉毒素 B1 (AFB1) 检测方法。该方法依赖于用 DNA 修饰的金纳米粒子 (NP) 编码的多克隆抗体和修饰的磁性微粒 (MMP) 的单克隆抗体，随后使用荧光定量聚合酶链反应 (FQ- PCR)检测方法。首先，制备用AFB1特有的DNA编码的NP探针，制备具有特异性结合AFB1的单克隆抗体的MMP探针。然后，获得 MMP-AFB1-NP 夹心化合物，通过 FQ-PCR 检测识别从 NP 释放的寡核苷酸序列，对纳米颗粒表面上的寡核苷酸进行去杂交，从而确定 AFB1 的存在。建立了检测AFB1的生物条码技术体系，灵敏度限值约为10-8 ng/mL，可与ELISA检测相同的靶点进行比较，表明我们可以用生物条码检测低阿摩尔水平的AFB1。条形码放大方法。这也是生物条形码类型检测中草药中 AFB1 的首次演示。