Ciguatera fish poisoning is a food-borne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the Gambierdiscus genus. Since most surveillance programs currently rely on the survey of Gambierdiscus cell densities and species composition, supplementary toxin-based methods allowing the time- and spatially integrated sampling of toxins in ciguateric environments are needed for a more reliable assessment and management of the risks associated with Gambierdiscus proliferation. Solid Phase Adsorption Toxin Tracking (SPATT) filters use porous synthetic resins capable of adsorbing toxins directly from the water column. To assess the ability of these passive monitoring devices to retain Gambierdiscus toxins, SPATT bags filled with 10 g of HP20 resin were deployed for 48 h in two French Polynesian locations at high (Nuku Hiva Island) vs. low to moderate (Kaukura Atoll) risk of ciguatera. CTXs could be detected in SPATT bags extracts from Nuku Hiva Island, as assessed by the mouse neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography – tandem mass spectrometry (LC–MS/MS) analyses. Results of in vitro experiments suggest that the saturation limit of CTXs on HP20 resin, for a deployment time of 48 h, is ≃ 55 ng P-CTX-3C equiv. g⁻¹ resin. Despite the non detection of maitotoxin (MTX), LC–MS/MS analyses showed that two other compounds also produced by Gambierdiscus species were retained on SPATT bags, i.e. iso-P-CTX-3B/C and a putative MTX analogue, known as MTX-3. This study, the first to demonstrate the suitability of SPATT technology for the in situ monitoring of Gambierdiscus toxins, highlights the potential application of this tool for routine ciguatera risk assessment and management programs.

Ciguatera鱼中毒是由食源性疾病引起的，其原因是食用了由Gambierdiscus属中鞭毛鞭毛虫产生的Ciguatoxins（CTX）污染的海鲜。由于大多数监视程序当前都依赖于冈比亚鞭毛虫细胞密度和物种组成的调查，因此需要基于毒素的补充方法，以便在严寒环境中对毒素进行时间和空间综合采样，以便更可靠地评估和管理与冈比亚鞭毛虫相关的风险增殖。固相吸附毒素追踪（SPATT）过滤器使用能够直接从水柱中吸附毒素的多孔合成树脂。评估这些被动监视设备的保留能力Gambierdiscus毒素，装满10克HP20树脂的SPATT袋在法属波利尼西亚的两个地点部署了48小时，地点为高（Nuku Hiva Island）与低至中度（Kaukura Atoll）发生卡加泰拉的风险。通过基于小鼠神经母细胞瘤细胞的测定（CBA-N2a）和液相色谱-串联质谱（LC-MS / MS）分析，可以在Nuku Hiva Island的SPATT袋提取物中检测到CTX。结果体外实验表明，CTXS对HP20树脂饱和极限，为48小时部署时间，是≃55纳克P-CTX-3C当量。g ^-1树脂。尽管未检测到人毒素（MTX），LC-MS / MS分析显示甘比氏双歧杆菌也产生了另外两种化合物物种被保留在SPATT袋中，即iso-P-CTX-3B / C和推定的MTX类似物（称为MTX-3）。这项研究首次证明了SPATT技术适用于就地监测甘比菌毒素的适用性，并着重指出了该工具在常规雪茄风险评估和管理计划中的潜在应用。