Wound therapy remains a clinical challenge due to the complexity of healing pathology and high demand of achieving functional and aesthetically satisfactory scars. Newly formed blood vessels are essential for tissue repair since they can support cells at the wound site with nutrition and oxygen. In this study, we investigated the effects of Asperosaponin VI (ASA VI) isolated from a traditional Chinese medicine, the root of Dipsacus asper Wall, in promoting angiogenesis, as well as its function in wound therapeutics. Treatment of human umbilical vein endothelial cells (HUVECs) with ASA VI (20-80 μg/mL) dose-dependently promoted the proliferation, migration and enhanced their angiogenic ability in vitro, which were associated with the up-regulated HIF-1α/VEGF signaling. Full-thickness cutaneous wound model rats were injected with ASA VI (20 mg·kg^-1·d^-1, iv) for 21 d. Administration of ASA VI significantly promoted the cutaneous wound healing, and more blood vessels were observed in the regenerated tissue. Due to rapid vascularization, the cellular proliferation status, granulation tissue formation, collagen matrix deposition and remodeling processes were all accelerated, resulting in efficient wound healing. In summary, ASA VI promotes angiogenesis of HUVECs in vitro via up-regulating the HIF-1α/VEGF pathway, and efficiently enhances the vascularization in regenerated tissue and facilitates wound healing in vivo. The results reveal that ASA VI is a potential therapeutic for vessel injury-related wounds.

中文翻译：

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Asperosaponin VI 通过上调 HIF-1α/VEGF 信号传导促进大鼠血管生成并加速伤口愈合。

由于愈合病理的复杂性以及对获得功能性和美观性令人满意的疤痕的高要求，伤口治疗仍然是临床挑战。新形成的血管对于组织修复至关重要，因为它们可以为伤口部位的细胞提供营养和氧气。在这项研究中，我们研究了从中药黄连根中分离出的白皂苷 VI (ASA VI) 在促进血管生成方面的作用，以及其在伤口治疗中的作用。用 ASA VI (20-80 μg/mL) 处理人脐静脉内皮细胞 (HUVECs) 剂量依赖性地促进增殖、迁移并增强其体外血管生成能力，这与上调 HIF-1α/VEGF 相关信令。全层皮肤创面模型大鼠注射ASA VI（20 mg·kg^-1 ·d ^-1 , iv) 21 天。ASA VI 的给药显着促进了皮肤伤口的愈合，并且在再生组织中观察到了更多的血管。由于快速血管化，细胞增殖状态、肉芽组织形成、胶原基质沉积和重塑过程都被加速，从而导致有效的伤口愈合。总之，ASA VI 通过上调 HIF-1α/VEGF 途径促进体外 HUVEC 的血管生成，并有效增强再生组织中的血管化并促进体内伤口愈合。结果表明，ASA VI 是治疗血管损伤相关伤口的潜在疗法。