Using RAPD and ISSR Molecular Markers to Analyze the Genetic Diversity of Rose scented Pelargonium Populations,Flavour and Fragrance Journal

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Using RAPD and ISSR Molecular Markers to Analyze the Genetic Diversity of Rose scented Pelargonium Populations
Flavour and Fragrance Journal ( IF 2.1 ) Pub Date : 2017-10-25 , DOI: 10.1002/ffj.3427
Qing-yuan Yi ₁ , Shao-wen Yi ₂ , Tai-feng Qin ₁ , Cheng-yun Li ₃

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In the field, the chemical constituents of the essential oil of Pelargonium graveolens cutting (diploid) and polyploid seedlings are the same, but the principal components of geraniol, geranyl formate, and linalool in polyploid plants are remarkably higher than those of cutting seedlings. The contents of citronellol, citronellyl formate, and rose oxide are significantly lower in polyploid seedlings than in cutting seedlings. ISSR and RAPD molecular markers were used to analyze P. graveolens in two different genetic populations and to compare the genetic background of differences between cutting and polyploid seedlings. The genomic DNA of 60 cutting and polyploid seedlings was extracted for PCR amplification by using 3 ISSR primers and 11 RAPD primers for random sampling from adjacent cultivated fields. Among the primers, ISSR‐25 primer detected the highest diversity of the loci. Seven loci of variation were found in the two populations. The variation rates detected using 3 ISSR primers were 13.3% and 6.7% for the cutting and polyploid populations, respectively. Genetic polymorphisms in P. graveolens genome with mutation rates of 11.7% and 3.3% were respectively determined in cutting and polyploid seedlings by using 3 RAPD primers (B11, P4, and R19). Both types of molecular markers also revealed the mutation in cutting and polyploid seedlings. Results demonstrated that a genetic difference can be observed by the molecular markers, although no evident phenotypic difference exists in cutting and polyploid seedlings in fields. The trend of these molecular markers is consistent, that is, individual genetic differences in cutting seedlings are greater than those in polyploid seedlings. These results suggested that polyploid seedlings in a population are relative uniform and applicable to production.

中文翻译：

使用RAPD和ISSR分子标记分析玫瑰香味天竺葵种群的遗传多样性

在田间，香叶天竺葵插条（二倍体）和多倍体幼苗的精油化学成分相同，但多倍体植物中香叶醇，香叶基香叶酯和芳樟醇的主要成分显着高于插条植物。多倍体幼苗中香茅醇，甲磺酸香茅酯和氧化玫瑰的含量显着低于cutting插幼苗。ISSR和RAPD分子标记用于分析重症疟原虫在两个不同的遗传种群中进行比较，比较cutting插和多倍体幼苗之间遗传差异的遗传背景。通过使用3个ISSR引物和11个RAPD引物从邻近的耕地中随机取样，提取了60棵插穗和多倍体幼苗的基因组DNA，以进行PCR扩增。在这些引物中，ISSR-25引物检测到了最高的基因座多样性。在两个种群中发现了七个变异位点。使用3个ISSR引物检测到的切割和多倍体群体的变异率分别为13.3％和6.7％。砾石拟南芥的遗传多态性通过使用3种RAPD引物（B11，P4和R19），分别在cutting插和多倍体幼苗中确定突变率分别为11.7％和3.3％的基因组。两种类型的分子标记物也揭示了cutting插和多倍体幼苗中的突变。结果表明，尽管在田间的cutting插和多倍体苗中没有明显的表型差异，但可以通过分子标记观察到遗传差异。这些分子标记的趋势是一致的，即cutting插幼苗的个体遗传差异大于多倍体幼苗的个体遗传差异。这些结果表明，种群中的多倍体幼苗相对均匀并且可用于生产。

更新日期：2017-10-25

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