当前位置: X-MOL 学术Chem. Sci. › 论文详情
Single quantum dot-based nanosensor for sensitive detection of 5-methylcytosine at both CpG and non-CpG sites
Chemical Science ( IF 9.063 ) Pub Date : 2017-12-13 , DOI: 10.1039/C7SC04813K
Zi-yue Wang, Li-juan Wang, Qianyi Zhang, Bo Tang, Chun-yang Zhang

DNA methylation is an important epigenetic modification in human genomes. Herein, we develop a single quantum dot (QD)-based nanosensor for sensitive detection of DNA methylation at both CpG and non-CpG sites using tricyclic ligation chain reaction (LCR)-mediated QD-based fluorescence resonance energy transfer (FRET). We design two sets of DNA probes (X and Y, X′ and Y′) for methylated DNA assay. In the presence of thermostable DNA ligase, probes X and Y may adjacently hybridize with the methylated DNA to obtain the ligated XY products which may function as the templates for probes X′ and Y′ to generate the X′Y′ products. The resultant X′Y′ products may in turn act as the templates to ligate probes X and Y for the generation of XY products, consequently inducing tricyclic LCR amplification under thermal denaturation conditions to generate a large number of XY products. The subsequent hybridization of XY products with the capture and reporter probes results in the formation of sandwich hybrids which may assemble on the 605QD surface to obtain 605QD–oligonucleotide–Cy5 nanostructures, inducing efficient FRET from the 605QD to Cy5 and the emission of Cy5. This nanosensor can detect DNA methylation at single 5-methylcytosine (5-mC) resolution with a detection limit of as low as 1.0 aM and a large dynamic range of 7 orders of magnitude. Moreover, this nanosensor can distinguish as low as a 0.01% methylation level, and it can detect DNA methylation in human lung cancer cells as well, holding great potential for accurate epigenetic evaluation and early cancer diagnosis.
更新日期:2018-01-31

 

分享到
评论: 0
期刊列表
广州大学胡春教授团队招聘科研秘书和特聘工程师
深圳格拉布斯研究院研究序列教授和工程师招聘启事
北京理工大学青年千人课题组联合招聘硕士生、博士生以及博士后人员(合成化学和材料方向)
天合科研
肠道菌群相关课题设计及研究策略专题会议
会议通知 | ACS全球科技研讨会:化学与生命科学前沿
【问答】请问如何表征金属纳米颗粒表面的缺陷?
2017年中科院JCR分区化学大类列表
试剂库存管理
化合物查询
down
wechat
bug