One-step eco-friendly approach for the fabrication of synergistically engineered fluorescent copper nanoclusters: sensing of Hg2+ ion and cellular uptake and bioimaging properties†,New Journal of Chemistry

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One-step eco-friendly approach for the fabrication of synergistically engineered fluorescent copper nanoclusters: sensing of Hg2+ ion and cellular uptake and bioimaging properties†
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2017-12-13 00:00:00 , DOI: 10.1039/c7nj04031h
Jigna R. Bhamore _{1,

2,

3,

4} , Balaji Deshmukh _{4,

5,

6,

7,

8} , Varun Haran _{4,

5,

6,

7,

8} , Sanjay Jha _{4,

9,

10,

11} , Rakesh Kumar Singhal _{4,

12,

13,

14} , Nibedita Lenka _{4,

5,

6,

7,

8} , Suresh Kumar Kailasa _{1,

2,

3,

4} , Z. V. P. Murthy _{2,

4,

11,

15}

Affiliation

Herein, a single-step eco-friendly synthetic method was established for the fabrication of synergistically engineered fluorescent copper nanoclusters (Cu NCs) using curcuma root (Curcuma longa L.) extract (curcuminoids) as a template. These Cu NCs are water-soluble and emitted bright blue fluorescence under UV light illumination at 365 nm. The synergistically engineered Cu NCs exhibited an emission peak at 440 nm with quantum yield of 7.2%. Due to their optical properties, a new fluorescent analytical strategy has been developed for the sensing of Hg²⁺ ions and used to evaluate cellular uptake and bioimaging properties on cancer cells (RIN-5F and MDAMB231) and fungal cells (Penicillium citrinum). It was interestingly observed that Hg²⁺ ion effectively quenched the emission peak of Cu NCs at 440 nm, which indicates that Cu NCs act as a fluorescent sensor. Taking advantage of this, a novel fluorescent Hg²⁺ sensor has been established, which showed a linear range of 0.0005–25 μM with a detection limit of 0.12 nM at room temperature. Furthermore, Cu NCs exhibited good selectivity for Hg²⁺ against other inorganic species, and the potentiality of the method was demonstrated by detecting Hg²⁺ ions in water samples. Furthermore, Cu NCs act as probes for imaging of two cancer cells (RIN-5F and MDAMB231) and fungal cells (Penicillium citrinum), and the cell viability results reveals their nontoxic nature.

中文翻译：

协同设计荧光铜纳米簇的一步式环保方法：检测Hg ²⁺离子以及细胞摄取和生物成像特性†

在此，建立了一种以一步一步的环保合成方法，以姜黄根（Curcuma longa L.）提取物（姜黄素）为模板，协同制备荧光铜纳米簇（Cu NCs）。这些Cu NCs是水溶性的，在365 nm的紫外线照射下发出亮蓝色荧光。协同设计的Cu NCs在440 nm处显示出发射峰，量子产率为7.2％。由于它们的光学特性，已开发出一种新的荧光分析策略来检测Hg ²⁺离子，并用于评估癌细胞（RIN-5F和MDAMB231）和真菌细胞（柠檬青霉）的细胞摄取和生物成像特性。有趣的是，观察到Hg ²⁺离子有效地淬灭了440 nm处Cu NCs的发射峰，这表明Cu NCs充当荧光传感器。利用这一优势，已建立了新型荧光Hg ²⁺传感器，该传感器的线性范围为0.0005–25μM，在室温下的检测极限为0.12 nM。此外，Cu NCs对Hg ²⁺表现出对其他无机物的良好选择性，并且通过检测水样中的Hg ²⁺离子证明了该方法的潜力。此外，Cu NCs用作两种癌细胞（RIN-5F和MDAMB231）和真菌细胞（柠檬青霉）成像的探针，细胞活力结果揭示了它们的无毒性质。

更新日期：2017-12-13

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