Signaling molecules, including H₂S and CO, are involved in a complex interplay within cells to maintain cellular homeostasis. In order to investigate the intracellular interplay of different gasotransmitters, new molecular tools are needed that combine the sensing and release capabilities of different small molecules in a single, multifunctional, and highly regulated molecular platform. This report gives the first example of a combined H₂S‐sensor/CO‐releasing molecule. This flavonol‐based molecular tool operates intracellularly via a highly regulated AND logic gated input–output sequence and provides fluorescent feedback for the H₂S detection and CO release steps. This linear sequence can be combined with a fluorescent molecular sensor for CO detection via a third distinct emission. Overall, this is the first molecular framework that can combine the sensing of H₂S with the controlled release of CO, two gaseous molecules that are known to exhibit reciprocal regulation and have overlapping targets in cellular environments.

中文翻译：

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通过逻辑门操作的 H2S 传感/CO 释放黄酮醇


信号分子（包括 H ₂ S 和 CO）参与细胞内复杂的相互作用，以维持细胞稳态。为了研究不同气体递质的细胞内相互作用，需要新的分子工具，将不同小分子的传感和释放能力结合在一个单一、多功能和高度调控的分子平台中。该报告给出了 H ₂ S 传感器/CO 释放分子组合的第一个例子。这种基于黄酮醇的分子工具通过高度调节的 AND 逻辑门控输入输出序列在细胞内运行，并为 H ₂ S 检测和 CO 释放步骤提供荧光反馈。该线性序列可以与荧光分子传感器结合，通过第三次不同的发射来检测 CO。总体而言，这是第一个能够将 H ₂ S 的传感与 CO 的受控释放相结合的分子框架，这两种气体分子已知表现出相互调节，并且在细胞环境中具有重叠的目标。