A powerful tool kit for recombinant protein expression has been developed by using wild‐type P. pastoris. A diverse spectrum of proteins and enzymes has been successfully produced by extracellular expression. Cytoplasmic expression and cell‐surface display created whole‐cell biocatalysts for biotransformation reactions, while metabolically engineered strains were used as microbial cell factories to produce value‐added chemicals.

中文翻译：

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毕赤酵母中的异源蛋白表达：最新研究进展与应用

利用野生型巴斯德毕赤酵母已经开发出了强大的重组蛋白表达工具套件。通过细胞外表达已成功产生了多种多样的蛋白质和酶。细胞质表达和细胞表面展示为生物转化反应创造了全细胞生物催化剂，而代谢工程菌株被用作微生物细胞工厂来生产增值化学品。