Recruitment and activation of brite (or beige) adipocytes has been advocated as a potential avenue for manipulating whole-body energy expenditure. Despite numerous studies illustrating the differences in gene and protein markers between brown, brite and white adipocytes, there is very little information on the adrenergic regulation and function of these brite adipocytes. We have compared the functional (cyclic AMP accumulation, oxygen consumption rates, mitochondrial function, glucose uptake, extracellular acidification rates, calcium influx) profiles of mouse adipocytes cultured from three contrasting depots, namely interscapular brown adipose tissue, and inguinal or epididymal white adipose tissues, following chronic treatment with the peroxisome proliferator-activated receptor γ (PPARγ) agonist rosiglitazone. Prototypical brown adipocytes readily express β₃-adrenoceptors, and β₃-adrenoceptor stimulation increases cyclic AMP accumulation, oxygen consumption rates, mitochondrial function, glucose uptake, and extracellular acidification rates. Treatment of brown adipocytes with rosiglitazone increases uncoupling protein 1 (UCP1) levels, and increases β₃-adrenoceptor mitochondrial function but does not affect glucose uptake responses. In contrast, inguinal white adipocytes only express UCP1 and β₃-adrenoceptors following rosiglitazone treatment, which results in an increase in all β₃-adrenoceptor-mediated functions. The effect of rosiglitazone in epididymal white adipocytes, was much lower compared to inguinal white adipocytes. Rosiglitazone also increased α₁-adrenoceptor mediated increases in calcium influx and glucose uptake (but not mitochondrial function) in inguinal and epididymal white adipocytes. In conclusion, the PPARγ agonist rosiglitazone promotes the induction and function of brite adipocytes cultured from inguinal and epididymal white adipose depots.

提倡激活和激活英国（或米色）脂肪细胞是操纵全身能量消耗的潜在途径。尽管有大量研究表明棕色，英国血脂和白色脂肪细胞之间基因和蛋白质标志物的差异，但是关于这些英国血脂细胞的肾上腺素调节和功能的信息很少。我们已经比较了从三个对比仓库（肩s间棕色脂肪组织和腹股沟或附睾白色脂肪组织）培养的小鼠脂肪细胞的功能（循环AMP积累，耗氧率，线粒体功能，葡萄糖摄取，细胞外酸化率，钙内流）配置文件在用过氧化物酶体增殖物激活的受体γ（PPARγ）激动剂罗格列酮进行慢性治疗后。₃肾上腺素能受体，β ₃ -肾上腺素能受体刺激增加环AMP累积，氧的消耗率，线粒体功能，葡萄糖摄取和胞外酸化率。罗格列酮增加解偶联蛋白1（UCP1）的水平，并增加β褐色脂肪细胞治疗₃肾上腺素受体线粒体的功能，但并不影响葡萄糖摄取的响应。与此相反，腹股沟白色脂肪细胞只表达UCP1和β ₃以下罗格列酮治疗肾上腺素能受体，这导致增加在所有β ₃ -肾上腺素能受体介导的功能。与腹股沟白色脂肪细胞相比，罗格列酮在附睾白色脂肪细胞中的作用要低得多。罗格列酮也增加α ₁-肾上腺素能受体介导的腹股沟和附睾白色脂肪细胞中钙内流和葡萄糖摄取（但不是线粒体功能）的增加。总之，PPARγ激动剂罗格列酮可促进腹股沟和附睾白色脂肪库培养的英国脂肪细胞的诱导和功能。