In this review, a number of systems are described to demonstrate the effect of polyelectrolyte chain stiffness (persistence length) on the coacervation phenomena, after we briefly review the field. We consider two specific types of complexation/coacervation: in the first type, DNA is used as a fixed substrate binding to flexible polyions such as gelatin A, bovine serum albumin and chitosan (large persistence length polyelectrolyte binding to low persistence length biopolymer), and in the second case, different substrates such as gelatin A, bovine serum albumin, and chitosan were made to bind to a polyion gelatin B (low persistence length substrate binding to comparable persistence length polyion). Polyelectrolyte chain flexibility was found to have remarkable effect on the polyelectrolyte-protein complex coacervation. The competitive interplay of electrostatic versus surface patch binding (SPB) leading to associative interaction followed by complex coacervation between these biopolymers is elucidated. We modelled the SPB interaction in terms of linear combination of attractive and repulsive Coulombic forces with respect to the solution ionic strength. The aforesaid interactions were established via a universal phase diagram, considering the persistence length of polyion as the sole independent variable.

在这篇综述中，在我们简要回顾了该领域之后，描述了许多系统来证明聚电解质链刚度（持续长度）对凝聚现象的影响。我们考虑两种特定类型的络合/凝聚：在第一种类型中，DNA被用作与柔性聚离子（例如明胶A，牛血清白蛋白和壳聚糖）结合的固定底物（大持久性聚电解质与低持久性生物聚合物结合），以及在第二种情况下，使不同的底物（例如明胶A，牛血清白蛋白和壳聚糖）与聚离子明胶B结合（低持久性底物与相当的持久性聚离子结合）。发现聚电解质链的柔性对聚电解质-蛋白质复合物的凝聚有显着影响。阐明了静电对表面贴剂结合（SPB）的竞争相互作用，这些相互作用导致缔合相互作用，随后这些生物聚合物之间发生复杂的凝聚。我们根据溶液离子强度的吸引力和排斥库仑力的线性组合对SPB相互作用进行建模。通过将聚离子的持续长度作为唯一的独立变量，通过通用相图建立了上述相互作用。