Objective

The objectives of the study were to evaluate the ability of a 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide (EDC)-containing primer to improve immediate bond strength of either self-etch or etch-and-rinse adhesive systems and to stabilize the adhesive interfaces over time. A further objective was to investigate the effect of EDC on the dentinal MMPs activity using zymographic analysis.

Methods

Freshly extracted molars (n = 80, 20 for each group) were selected to conduct microtensile bond strength tests. The following groups were tested, immediately or after 1-year aging in artificial saliva: G1: Clearfil SE (CSE) primer applied on unetched dentin, pretreated with 0.3 M EDC water-solution for 1 min and bonded with CSE Bond; G2: as G1 but without EDC pre-treatment; G3: acid-etched (35% phosphoric-acid for 15s) dentin pretreated with 0.3 M EDC, then bonded with XP Bond (XPB); Group 4 (G4): as G3 without EDC pre-treatment. Further, gelatinase activity in dentin powder treated with CSE and XPB with and without EDC pre-treatment, was analyzed using gelatin zymography.

Results

The use of 0.3 M EDC-containing conditioner did not affect the immediate bond strength of XPB or CSE adhesive systems (p > 0.05), while it improved the bond strength after 1 year of aging (p < 0.05). Pre-treatment with EDC followed by the application of CSE resulted in an incomplete MMPs inactivation, while EDC pretreatment followed by the application of XPB resulted in an almost complete inactivation of dentinal gelatinases.

Significance

The μTBS and zymography results support the efficacy of EDC over time and reveal that changes within the dentin matrix promoted by EDC are not adhesive-system-dependent.

中文翻译：

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交联作用对牙本质结合强度和MMPs活性的影响

客观的

该研究的目的是评估含1-乙基-3（3-二甲基氨基丙基）碳二亚胺（EDC）的底漆改善自蚀刻或蚀刻和漂洗粘合剂体系的即时粘结强度并稳定化的能力。随着时间的流逝，粘合剂界面会发生变化。另一个目的是使用酶谱分析法研究EDC对牙本质MMPs活性的影响。

方法

选择新鲜提取的磨牙（每组n = 80，每组20个）进行微拉伸粘合强度测试。在人造唾液中立即老化或在老化1年后测试以下组：G1：Clearfil SE（CSE）底漆涂在未蚀刻的牙本质上，用0.3 M EDC水溶液预处理1分钟并用CSE Bond粘结；G2：与G1相同，但未进行EDC预处理；G3：用0.3M EDC预处理的酸蚀牙本质（35％磷酸，持续15s），然后用XP Bond（XPB）粘合；第4组（G4）：未经EDC预处理的G3。此外，使用明胶酶谱分析法分析了在有和没有EDC预处理的情况下，经CSE和XPB处理过的牙本质粉中的明胶酶活性。

结果

使用含0.3 M EDC的调理剂不会影响XPB或CSE粘合剂体系的即时粘合强度（p> 0.05），而在老化1年后可以提高粘合强度（p <0.05）。EDC预处理后再应用CSE导致MMPs失活不完全，而EDC预处理后再应用XPB导致牙本质明胶酶几乎完全失活。

意义

μTBS和酶谱学结果支持了EDC随时间推移的功效，并揭示了EDC促进的牙本质基质内的变化与黏附剂系统无关。