Effects of benzophenone-3 (BP-3) on the green alga, Chlamydomonas reinhardtii, and the cyanobacterium, Microcystis aeruginosa, were investigated. The tested organisms were exposed to environmental levels of BP-3 for 10 days, at nominal concentrations from 0.01 to 5000 μg L⁻¹. Specific growth rate and photosynthetic pigments were employed to evaluate the toxic responses. The two tested algae had distinct toxic responses towards BP-3 stress, with the green alga C. reinhardtii being more sensitive than the cyanobacterium M. aeriginosa, based on EC₂₀ and EC₅₀ values. Uptake of BP-3 from the medium occurred in both species, with M. aeruginosa showing greater overall uptake (27.2–77.4%) compared to C. reinhardtii (1.1–58.4%). The effects of BP-3 on C. reinhardtii were variable at concentrations lower than 100 μg L⁻¹. At higher concentrations, the specific growth rate of C. reinhardtii decreased following a reduction in chlorophyll a (chl-a) content. Further experiments showed that BP-3 regulated the growth of C. reinhardtii by affecting the production of chl-a, chlorophyll b and carotenoids. In M. aeruginosa, specific growth rate was only moderately affected by BP-3. Additionally, the production of chl-a was significantly inhibited over the different exposure concentrations, while the production of carotenoids was stimulated. These results indicate a potential detrimental effect on prokaryotes and eukaryotes and that the mechanism of action varies with species.

研究了二苯甲酮3（BP-3）对绿藻莱茵衣藻和蓝藻细菌铜绿微囊藻的影响。被测生物以标称浓度0.01至5000μgL ^-1暴露于BP-3环境水平10天。使用特定的生长速率和光合色素来评估毒性反应。两种试验藻类具有明显的毒性反应朝BP-3应力，与绿藻莱茵衣藻比所述蓝细菌更敏感M. aeriginosa，基于EC ₂₀和EC _50个值。两种物种均从培养基中吸收了BP-3 。示出相比更大的总摄取（27.2-77.4％）莱茵衣藻（1.1-58.4％）。BP-3对莱茵衣藻的影响在浓度低于100μgL ^-1时是可变的。在较高的浓度下，随着叶绿素a（chl-a）含量的减少，莱茵衣藻的比生长速率降低。进一步的实验表明，BP-3通过影响chl-a，叶绿素b和类胡萝卜素的产生来调节莱茵衣藻的生长。在铜绿假单胞菌，比生长率仅受BP-3的中等影响。另外，在不同的暴露浓度下，chl-a的产生被显着抑制，而类胡萝卜素的产生受到刺激。这些结果表明对原核生物和真核生物的潜在有害作用，并且作用机理随物种而变化。