Abstract A new way of real-time preparation of surface enhanced Raman scattering (SERS) substrate was developed by laser photoreduction of AgNO3 mixed with probe molecules (aromatic amine, aromatic mercaptan or phenolic compound) in capillary. The preparation of Ag nanoparticle cluster and acquisition of SERS signal could be performed synchronously and finished within several seconds. Probe molecules were analyzed on line in capillary. It was proposed that the rapid photoreduction process was initiated by photoinduced electron transfer from electron donors aromatic molecule to Ag+ under laser illumination. The p electrons from N (S or O) and π electrons from aromatic ring could form p-π conjugated structure. These delocalized electrons assisted the rapid reduction of Ag+ in such a short time. Additionally, Raman maps with 8 μm increment in X–Y directions (35 spectral spots) demonstrated good point-to-point repeatability of the substrate with the relative standard deviation less than 9%. Under optimal condition, several probe molecules, including benzidine, bisphenol A and so on were determined on line in capillary by this new method.

中文翻译：

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实时制备用于在线分析毛细管中芳香分子的表面增强拉曼散射基底

摘要 通过激光光还原 AgNO3 与探针分子（芳香胺、芳香硫醇或酚类化合物）在毛细管中的混合，开发了一种实时制备表面增强拉曼散射 (SERS) 基底的新方法。Ag纳米粒子簇的制备和SERS信号的采集可以同步进行，并在几秒钟内完成。在毛细管中在线分析探针分子。有人提出，快速光还原过程是通过在激光照射下从电子供体芳香分子到 Ag+ 的光诱导电子转移而引发的。来自N（S或O）的p电子和来自芳环的π电子可以形成p-π共轭结构。这些离域电子有助于在如此短的时间内快速还原 Ag+。此外，在 X-Y 方向上以 8 μm 增量（35 个光谱点）的拉曼图显示了基板的良好点对点重复性，相对标准偏差小于 9%。在最佳条件下，该新方法在毛细管中在线测定了联苯胺、双酚A等多种探针分子。