A reversed phase liquid chromatographic (RPLC) method was developed to simultaneously detect and quantify creatinine, quinolinic acid, gentisic acid and 4-hydroxybenzoic acid in urine. These four bio-markers are present in relatively high concentrations in urine. Using a 5% methanol in water mobile phase with 0.6% acetic acid and a Zorbax C18 column, baseline resolution for all four biomarkers in synthetic urine was achieved. Better resolution was obtained for the separation of these four compounds when water rich mobile phases were used. Detection of the four biomarkers in urine using the proposed RPLC method is limited by background from the urine matrix for the later eluting compounds and from the dead marker for earlier eluting compounds.

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测定尿液中龙胆酸和相关肾细胞癌生物标志物的高效液相色谱方法的开发和验证

开发了一种反相液相色谱 (RPLC) 方法来同时检测和定量尿液中的肌酐、喹啉酸、龙胆酸和 4-羟基苯甲酸。这四种生物标志物在尿液中的浓度相对较高。使用 5% 甲醇水溶液和 0​​.6% 乙酸的流动相和 Zorbax C18 色谱柱，实现了合成尿中所有四种生物标志物的基线分离度。当使用富水流动相时，这四种化合物的分离获得了更好的分离度。使用所提出的 RPLC 方法检测尿液中的四种生物标志物受到来自尿液基质中较晚洗脱化合物的背景和来自较早洗脱化合物的死亡标志物的背景的限制。