Abstract A rapid, simple, sensitive and ultrafast capillary electrophoretic method – large-volume sample stacking (LVSS) without a polarity switching step has been developed in this study for determining ammonia and 9 amines, including ethanolamine, cyclohexylamine, hydrazine, morpholine, triethanolamine, and dimethylamine in steam water and their degradation products, such as methylamine, ethylamine, diethanolamine, and ammonia. The background electrolyte (BGE) containing acridine as a probe was firstly used for indirect UV detection. Acridine was shown to be very beneficial for the determination of low molecular mass amines due to its high electrophoretic mobility and at the same time high molar absorption coefficient (e252) of 1.7 ∗ 105. No laborious sample preparation was needed. Separation and detection was achieved in about 8.5 min for BGE containing acridine per sample. Limits of detection (LOD) using acridine were as low as 0.003 mg/L. The calibration plots showed good linearity over the concentration range from 0.01 to 1 mg/L for ammonia and the amines of interest for nuclear and thermal power plants. The proposed method was successfully applied to the determination of ethanolamine and cyclohexylamine in steam water samples obtained from thermal power plant and the analytical results were in good agreement with those obtained by reference ion chromatography (IC) method.

中文翻译：

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使用新型高吸收探针进行间接紫外检测，大体积样品堆积，用于通过 CE 分析蒸汽水中的低分子量胺

摘要 本研究开发了一种快速、简单、灵敏和超快的毛细管电泳方法——大体积样品堆积 (LVSS)，无需极性转换步骤，用于测定氨和 9 种胺，包括乙醇胺、环己胺、肼、吗啉、三乙醇胺、蒸汽水中的二甲胺及其降解产物，如甲胺、乙胺、二乙醇胺、氨等。以吖啶为探针的背景电解质（BGE）首次用于间接紫外检测。由于其高电泳迁移率和 1.7 * 105 的高摩尔吸光系数 (e252)，吖啶被证明对低分子量胺的测定非常有益。不需要费力的样品制备。8个左右就实现了分离和检测。每个样品含有吖啶的 BGE 需要 5 分钟。使用吖啶的检测限 (LOD) 低至 0.003 mg/L。校准图在 0.01 至 1 mg/L 的浓度范围内显示出良好的线性，对于氨和核电厂和热电厂的目标胺。该方法成功应用于火力发电厂蒸汽水样中乙醇胺和环己胺的测定，分析结果与参考离子色谱法(IC)得到的分析结果吻合较好。