Abstract In this work, four kinds of deep eutectic solvents (DESs), composed of choline chloride (ChCl) and several alcohols, were synthesized and characterized by 1H NMR and FT-IR spectra. Based on DES (ChCl and 1,4-butanediol) and inorganic salt solution (dipotassium phosphate, K2HPO4), aqueous two-phase system (ATPS) coupled with high-performance liquid chromatography has been developed and applied. This method was used for the efficient extraction and determination of eight common ginsenosides (including ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2 and Rd) in the traditional Chinese medicine injection named Kang'ai injection. To achieve optimum extraction performance, the experimental conditions were optimized, including type and amount of DES and salt, ultrasound time, temperature and pH value. In the dynamic linearity range, experimental results showed satisfactory recoveries of analytes ranging from 92.7–110.8% with the relative standard deviations (RSDs) ranging from 1.2–3.9%. It has been proved that DES-ATPS has great potential for determination of active ingredients in traditional Chinese medicine injection as well as samples in other analytical science field.

中文翻译：

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低共熔水两相萃取-HPLC-UV/DAD法测定康艾注射液中常见人参皂苷

摘要 在这项工作中，合成了四种由氯化胆碱（ChCl）和几种醇组成的低共熔溶剂（DESs），并通过 1H NMR 和 FT-IR 光谱对其进行了表征。基于DES（ChCl和1,4-丁二醇）和无机盐溶液（磷酸二钾，K2HPO4），开发并应用了水相两相系统（ATPS）与高效液相色谱联用。本方法用于中药注射剂康艾注射液中8种常见人参皂苷（包括人参皂苷Rg1、Re、Rf、Rg2、Rb1、Rc、Rb2和Rd）的高效提取和测定。为获得最佳提取性能，对实验条件进行了优化，包括DES和盐的类型和用量、超声时间、温度和pH值。在动态线性范围内，实验结果表明，分析物的回收率为 92.7-110.8%，相对标准偏差 (RSD) 为 1.2-3.9%。实践证明，DES-ATPS在中药注射剂中有效成分的测定以及其他分析科学领域的样品中具有巨大的潜力。