Endophytic bacteria occupy the same niche of phytopathogens and may produce metabolites that induce the host plant systemic resistance and growth. Host and environmental variables often determine the endophytic community’s structure and composition. In this study, we addressed whether the plant genotype, organ, and geographic location influence the structure, composition, and functionality of endophytic bacterial communities in Paullinia cupana. To characterize the communities and identify strains with potential application in agriculture, we analyzed two P. cupana genotypes cultivated in two cities of the State of Amazonas, Brazil. Endophytic bacteria were isolated from surface-disinfested root, leaf, and seed tissues through the fragmentation and maceration techniques. The colonization rate, number of bacteria, richness, diversity, and functional traits were determined. The plant growth-promoting ability of selected bacterial strains was assessed in Sorghum bicolor. We identified 95 bacterial species distributed in 29 genera and 3 phyla (Proteobacteria, Actinobacteria, and Firmicutes). The colonization rate, richness, diversity, and species composition varied across the plant organs; the last parameter also varied across the plant genotype and location. Some strains exhibited relevant plant growth-promoting traits and antagonistic traits against the main phytopathogens of P. cupana, but they were not separated by functional traits. The main bacterial strains with plant growth-promoting traits induced S. bicolor growth. Altogether, our findings open opportunities to study the application of isolated endophytic bacterial strains in the bioprospection of processes and products.

内生细菌占据植物病原体的相同位置，并可能产生代谢产物，从而诱导宿主植物的系统抗性和生长。寄主和环境变量通常决定内生群落的结构和组成。在这项研究中，我们探讨了植物基因型，器官和地理位置是否会影响Paullinia cupana中内生细菌群落的结构，组成和功能。为了描述社区特征并确定在农业上有潜在应用的菌株，我们分析了两个P. cupana在巴西亚马逊州两个城市种植的基因型。通过破碎和浸软技术从表面灭活的根，叶和种子组织中分离出内生细菌。确定定殖率，细菌数量，丰富度，多样性和功能性状。在双色高粱中评估了所选菌株的植物促生能力。我们确定了分布在29个属和3个门的95种细菌（变形杆菌，放线菌和Firmicutes）。不同植物器官的定殖率，丰富度，多样性和物种组成各不相同。最后一个参数在植物基因型和位置上也不同。一些菌株表现出相关的促进植物生长的性状和对铜绿假单胞菌主要植物病原体的拮抗性，但是它们并没有被功能性性状分开。具有促进植物生长性状的主要细菌菌株诱导双色链球菌生长。总之，我们的发现为研究分离的内生细菌菌株在工艺和产品的生物勘探中的应用提供了机会。