Colistin sulfate is widely used in both human and veterinary medicine. However, its effect on the microbial ecologyis unknown. In this study, we determined the effect of colistin sulfate on the diversity of soil microorganisms by amplified rDNA restriction analysis (ARDRA) and high-throughput sequencing.ARDRAshowed that the diversity of DNA from soil microorganisms was reduced after soil was treated with colistin sulfate, with the most dramatic reductionobserved after 35 days of treatment. High-throughput sequencing showed that the Chao1 and abundance-based coverage estimators (ACE) were reduced in the soils treated with colistin sulfate for 35 dayscompared to those treated with colistin sulfate for 7 days. Furthermore, Chao1 and ACE tended to be lower when higher concentration of colistin sulfate was used, suggesting that the microbial abundance is reduced by colistin sulfate in a dose-dependent manner. Shannon index showed that the diversity of soil microorganism was reduced upon treatment with colistin sulfate compared to the untreated control group. Following 7 days of treatment, Bacillus, Clostridiumand Sphingomonas were sensitive to all the concentration of colistin sulfate used in this study. Following 35 days of treatment, the abundance of Choroplast, Haliangium, Pseudomonas, Lactococcus, and Clostridium was significantly decreased. Our results demonstrated that colistin sulfate especially at high concentration (≥5 mg/kg) could alter the population structure of microorganisms and consequently the microbial community function in soil.

硫酸考司汀广泛用于人类和兽医医学。然而，其对微生物生态学的影响尚不清楚。在这项研究中，我们通过扩增rDNA限制性分析（ARDRA）和高通量测序确定了硫酸粘菌素对土壤微生物多样性的影响.ARDRA表明，用硫酸粘菌素处理土壤后，土壤微生物中DNA的多样性降低了，治疗35天后观察到最明显的减少。高通量测序表明，与硫酸粘菌素处理7天相比，硫酸粘菌素处理35天的土壤中Chao1和基于丰度的覆盖率估计值（ACE）降低。此外，当使用更高浓度的硫酸粘菌素硫酸盐时，Chao1和ACE趋于降低，这表明硫酸粘菌素降低了微生物的丰度，并呈剂量依赖性。香农指数表明，与未处理的对照组相比，硫酸粘杆菌素处理后土壤微生物的多样性降低了。经过7天的治疗，芽孢杆菌，梭状芽孢杆菌和鞘氨醇单胞菌对本研究中使用的所有硫酸粘菌素浓度均敏感。治疗35天后，Chorplast，Haliangium，Pseudomonas，Lactococcus和Clostridium的丰度明显降低。我们的结果表明，硫酸粘菌素特别是高浓度（≥5mg / kg）可以改变微生物的种群结构，从而改变土壤中的微生物群落功能。