Fusaric acid (FA) is a fungal metabolite produced by several Fusarium species responsible for wilts and root rot diseases of a great variety of plants. Bacillus spp. and Pseudomonas spp. have been considered as promising biocontrol agents against phytopathogenic Fusarium spp., however it has been demonstrated that FA negatively affects growth and production of some antibiotics in these bacteria. Thus, the capability to degrade FA would be a desirable characteristic in bacterial biocontrol agents of Fusarium wilt. Taking this into account, bacteria isolated from the rhizosphere of barley were screened for their ability to use FA as sole carbon and energy source. One strain that fulfilled this requirement was identified according to sequence analysis of 16S rRNA, gyrB and recA genes as Burkholderia ambifaria. This strain, designated T16, was able to grow with FA as sole carbon, nitrogen and energy source and also showed the ability to detoxify FA in barley seedlings. This bacterium also exhibited higher growth rate, higher cell densities, longer survival, higher levels of indole-3-acetic acid (IAA) production, enhanced biofilm formation and increased resistance to different antibiotics when cultivated in Luria Bertani medium at pH 5.3 compared to pH 7.3. Furthermore, B. ambifaria T16 showed distinctive plant growth-promoting features, such as siderophore production, phosphate-solubilization, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, in vitro antagonism against Fusarium spp. and improvement of grain yield when inoculated to barley plants grown under greenhouse conditions. This strain might serve as a new source of metabolites or genes for the development of novel FA-detoxification systems.

Fusaric acid（FA）是一种真菌代谢产物，由几种镰刀菌属物种产生，负责多种植物的枯萎和根腐病。芽孢杆菌属。和假单胞菌属。已被认为是对抗植物病原性镰刀菌的有前途的生物防治剂，但是已经证明FA对这些细菌中某些抗生素的生长和产生有负面影响。因此，降解镰刀菌的能力将是镰刀菌细菌生物防治剂的理想特性。枯萎。考虑到这一点，筛选了从大麦根际分离出的细菌将FA用作唯一碳和能源的能力。根据对16S rRNA，gyrB和recA基因的序列分析，鉴定出一种满足该要求的菌株为Burkholderia ambifaria。该菌株命名为T16，能够与FA一起生长，作为唯一的碳，氮和能源，并且还显示出对大麦幼苗中的FA进行解毒的能力。与pH值5.3相比，在Luria Bertani培养基中培养时，该细菌还表现出更高的生长速率，更高的细胞密度，更长的存活时间，更高的吲哚-3-乙酸（IAA）产生水平，增强的生物膜形成和对不同抗生素的抗性。 7.3。此外，冈比亚芽孢杆菌T16表现出独特的促进植物生长的功能，例如铁载体生成，磷酸盐增溶，1-氨基环丙烷-1-羧酸盐（ACC）脱氨酶活性，对镰刀菌的体外拮抗作用spp。接种到在温室条件下生长的大麦植物时，谷物产量提高了。该菌株可作为新的FA排毒系统开发的代谢物或基因的新来源。