Green synthetic route of silver nanoparticles (AgNPs) has already been proved to be an advantageous over other physico-chemical approaches due to its simplicity, cost effectiveness, ecofriendly and nontoxicity. In this finding, aqueous Wedelia chinensis leaf extract (WLE) mediated synthesis of AgNPs was approached. Surface plasmon resonance (SPR) band at 408 nm preliminary indicated the formation of AgNPs, while TEM and XRD characterization confirmed the formation of spherically shaped and crystalline AgNPs with an average size of 31.68 nm, respectively. The plausible biomolecules in the aqueous leaf extract responsible for the reduction and stabilization of AgNPs were identified by FTIR analysis and found to be polyphenolic groups in flavonoid. Further, synthesized AgNPs was explored for different biological applications. Biosynthesized AgNPs showed significant free radical scavenging activity as compared to Wedelia leaf extract and antibacterial activity against clinically isolated test pathogens where Gram-negative bacteria were found more susceptible to AgNPs than Gram-positive one. In addition, in vitro cytotoxic response was also evaluated on hepatocellular carcinoma Hep G2 cell lines and showed a dose-dependent cytotoxic response with an IC50 value of 25 μg/mL.

中文翻译：

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探索黄花菜叶辅助银纳米粒子在抗氧化、抗菌和体外细胞毒方面的应用

银纳米粒子 (AgNPs) 的绿色合成路线由于其简单、成本效益、环保和无毒等优点，已被证明优于其他物理化学方法。在这一发现中，接近了水性茯苓叶提取物 (WLE) 介导的 AgNPs 合成。408 nm 的表面等离子共振 (SPR) 带初步表明 AgNPs 的形成，而 TEM 和 XRD 表征分别证实了平均尺寸为 31.68 nm 的球形和结晶 AgNPs 的形成。通过 FTIR 分析鉴定了水叶提取物中负责减少和稳定 AgNPs 的似是而非的生物分子，并发现它们是类黄酮中的多酚基团。此外，针对不同的生物学应用探索了合成的 AgNPs。与 Wedelia 叶提取物相比，生物合成的 AgNPs 显示出显着的自由基清除活性，并且对临床分离的测试病原体具有抗菌活性，其中革兰氏阴性菌比革兰氏阳性菌更容易受到 AgNPs 的影响。此外，还评估了对肝细胞癌 Hep G2 细胞系的体外细胞毒性反应，并显示出剂量依赖性细胞毒性反应，IC50 值为 25 μg/mL。