当前位置: X-MOL 学术Mol. Cell. Neurosci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
ENA/VASP proteins regulate exocytosis by mediating myosin VI-dependent recruitment of secretory granules to the cortical actin network
Molecular and Cellular Neuroscience ( IF 2.6 ) Pub Date : 2017-08-07 , DOI: 10.1016/j.mcn.2017.07.005
Vanesa M. Tomatis , Peter Josh , Andreas Papadopulos , Rachel S. Gormal , Vanessa Lanoue , Sally Martin , Frédéric A. Meunier

In neurosecretory cells, myosin VI associated with secretory granules (SGs) mediates their activity-dependent recruitment to the cortical actin network and is necessary to sustain exocytosis. The mechanism by which myosin VI interacts with SGs is unknown. Using a myosin VI pull-down assay and mass spectrometry we identified Mena, a member of the ENA/VASP family, as a myosin VI binding partner in PC12 cells, and confirmed that Mena colocalized with myosin VI on SGs. Using a knock-sideways approach to inactivate the ENA/VASP family members by mitochondrial relocation, we revealed a concomitant redistribution of myosin VI. This was ensued by a reduction in the association of myosin VI with SGs, a decreased SG mobility and density in proximity to the plasma membrane as well as decreased evoked exocytosis. These data demonstrate that ENA/VASP proteins regulate SG exocytosis through modulating the activity of myosin VI.



中文翻译:

ENA / VASP蛋白通过介导肌球蛋白VI依赖性分泌颗粒向皮质肌动蛋白网络的募集来调节胞吐作用

在神经分泌细胞中,与分泌颗粒(SGs​​)相关的肌球蛋白VI介导其依赖于活性的募集到皮质肌动蛋白网络,并且对于维持胞吐作用是必需的。肌球蛋白VI与SG相互作用的机制尚不清楚。使用肌球蛋白VI下拉测定法和质谱法,我们鉴定了ENA / VASP家族成员Mena作为PC12细胞中的肌球蛋白VI结合伴侣,并确认Mena与肌球蛋白VI在SGs上共定位。使用侧向敲除方法通过线粒体重定位灭活ENA / VASP家庭成员,我们揭示了肌球蛋白VI的伴随重新分布。这是由于肌球蛋白VI与SG的缔合减少,SG迁移率和质膜附近密度降低以及诱发的胞吐作用降低所致。

更新日期:2017-08-07
down
wechat
bug