Application of an aqueous two-phase system (ATPS) coupled with ultrasonic technology for the extraction of phloridzin from Malus micromalus Makino was evaluated and optimized by response surface methodology (RSM). The ethanol/ammonium sulfate ATPS was selected for detailed investigation, including the phase diagram, effect of phase composition and extract conditions on the partition of phloridzin, and the recycling of ammonium sulfate. In addition, the evaluation of extraction efficiency and the identification of phloridzin were investigated. The optimal partition coefficient (6.55) and recovery (92.86%) of phloridzin were obtained in a system composed of 35% ethanol (w/w) and 16% (NH4 )2 SO4 (w/w), 51:1 liquid-to-solid ratio, and extraction temperature of 36 °C. Comparing with the traditional solvent extraction with respective 35% and 80% ethanol, ultrasonic-assisted aqueous two-phase extraction (UAATPE) strategy had significant advantages with lower ethanol consumption, less impurity of sugar and protein, and higher extracting efficiency of phloridzin. Our result indicated that UAATPE was a valuable method for the extraction and preliminary purification of phloridzin from the fruit of Malus micromalus Makino, which has great potential in the deep processing of Malus micromalus Makino industry to increase these fruits' additional value and drive the local economic development.

中文翻译：

---

乙醇/硫酸铵体系超声辅助水相两相萃取槭树根皮苷的工艺优化-【维普网】-仓储式在线作品出版平台-www.cqvip.com

应用响应面法 (RSM) 评估和优化水相两相系统 (ATPS) 结合超声波技术从小苹果 (Malus micromalus Makino) 中提取根皮苷。选择乙醇/硫酸铵ATPS进行详细研究，包括相图、相组成和提取条件对根皮苷分配的影响以及硫酸铵的回收利用。此外，还对提取效率的评价和根皮苷的鉴定进行了研究。根皮苷的最佳分配系数 (6.55) 和回收率 (92.86%) 在由 35% 乙醇 (w/w) 和 16% (NH4 )2 SO4 (w/w)、51:1 液比组成的体系中获得。 -固比，萃取温度为 36 °C。与分别使用35%和80%乙醇的传统溶剂萃取相比，超声辅助水相两相萃取(UAATPE)策略具有乙醇消耗低、糖和蛋白质杂质少、根皮苷提取效率高等显着优势。我们的研究结果表明，UAATPE是一种从牧野苹果果实中提取和初步纯化根皮苷的有价值的方法，在牧野苹果产业的深加工中具有很大的潜力，可以提高这些水果的附加值，推动当地经济发展。发展。