Abstract To increase information content per screen, a dual-wavelength, homologous and high-throughput fluorescence polarization immunoassay (DWFPIA) for simultaneous detection of total aflatoxins (AFs) and family zearalenones (ZENs) was developed. Aflatoxin B1 (AFB1) and zearalanone (ZAN) were labeled with different fluoresceins, and then combined with corresponding broad-specific antibodies to develop the DWFPIA. Under optimal conditions, the half maximal inhibitory concentrations of the FPIA in buffer were 2.68 μg/L and 4.08 μg/L for total four AFs (the weight ratio of AFB1: Aflatoxin B2: Aflatoxin G1: Aflatoxin G2 was 1: 1: 1: 1) and six ZEN analogs (the weight ratio of ZEN: ZAN: α-zearalenone: β-zearalenone: α-zearalanone: β-zearalanone was 1: 1: 1: 1: 1: 1) by mixing equal volumes (1: 1, v/v), respectively. The corresponding limit of detection values in maize flour samples were 4.98 μg/kg and 11.03 μg/kg, and the mean recoveries ranged from 78.6 to 103.6% with the coefficients of variation below 19.2%. The whole detection procedure, including sample preparation, took less than 30 min. Finally, the DWFPIA was applied to screen naturally contaminated maize flour samples, and the detection data were similar to those with HPLC-MS/MS.

中文翻译：

---

双波长荧光偏振免疫分析增加每个屏幕的信息含量：同时检测玉米中总黄曲霉毒素和玉米赤霉烯酮家族的应用

摘要 为了增加每个屏幕的信息含量，开发了一种双波长、同源和高通量荧光偏振免疫分析 (DWFPIA)，用于同时检测总黄曲霉毒素 (AFs) 和家族玉米赤霉烯酮 (ZENs)。黄曲霉毒素 B1 (AFB1) 和玉米赤霉烯酮 (ZAN) 用不同的荧光素标记，然后结合相应的广谱特异性抗体开发 DWFPIA。在最佳条件下，FPIA在缓冲液中的半数最大抑菌浓度分别为2.68 μg/L和4.08 μg/L，共4种AF（AFB1：黄曲霉毒素B2：黄曲霉毒素G1：黄曲霉毒素G2的重量比为1：1：1： 1) 和六种 ZEN 类似物（ZEN: ZAN: α-玉米赤霉烯酮: β-玉米赤霉烯酮: α-玉米赤霉烯酮: β-玉米赤霉烯酮的重量比为 1: 1: 1: 1: 1: 1）混合等体积 (1: 1: 1) 1，v/v)，分别。玉米粉样品的相应检出限分别为4.98 μg/kg和11.03 μg/kg，平均回收率为78.6%～103.6%，变异系数低于19.2%。整个检测过程，包括样品制备，耗时不到 30 分钟。最后，应用 DWFPIA 筛选天然污染的玉米粉样品，检测数据与 HPLC-MS/MS 相似。