Alphaproteobacterium strain MOLA1416, related to Mycoplana ramosa DSM 7292 and Chelativorans intermedius CC-MHSW-5 (93.6% 16S rRNA sequence identity) was isolated from the marine lichen, Lichina pygmaea and its chemical composition was characterized by a metabolomic network analysis using LC-MS/MS data. Twenty-five putative different compounds were revealed using a dereplication workflow based on MS/MS signatures available through GNPS (https://gnps.ucsd.edu/). In total, ten chemical families were highlighted including isocoumarins, macrolactones, erythrinan alkaloids, prodiginines, isoflavones, cyclohexane-diones, sterols, diketopiperazines, amino-acids and most likely glucocorticoids. Among those compounds, two known metabolites (13 and 26) were isolated and structurally identified and metabolite 26 showed a high cytotoxic activity against B16 melanoma cell lines with an IC50 0.6 ± 0.07 μg/mL.

中文翻译：

---

与海洋地衣 Lichina pygmaea 相关的 Alphaproteobacterium 菌株 MOLA1416 的化学分析

从海洋地衣 Lichina pygmaea 中分离出与 Mycoplana ramosa DSM 7292 和 Chelativorans intermedius CC-MHSW-5（93.6% 16S rRNA 序列同一性）相关的 Alphaproteobacterium 菌株 MOLA1416，并通过使用 LC-MS 的代谢组学网络分析表征其化学成分/MS 数据。使用基于可通过 GNPS (https://gnps.ucsd.edu/) 获得的 MS/MS 特征的去复制工作流程揭示了 25 种推定的不同化合物。总共强调了 10 个化学家族，包括异香豆素、大环内酯、赤藓聚糖生物碱、prodiginines、异黄酮、环己烷二酮、甾醇、二酮哌嗪、氨基酸和最可能的糖皮质激素。在这些化合物中，