Bisphenol A (BPA) is a pervasive environmental toxicant with known reproductive effects on sperm parameters and hormone levels. Several observational studies have investigated the associations between BPA exposure and male reproductive function, but findings are inconsistent. The objective of this study was to assess the associations between urinary BPA concentrations and semen quality and reproductive hormone levels in a cross-sectional study with 215 healthy young university students (18–23 years old), investigated between 2010 and 2011 in Southern Spain (Murcia Region). All subjects provided urine, blood serum and semen samples on a single day. Urinary BPA concentrations were measured by dispersive liquid–liquid microextraction and ultra-high performance liquid chromatography with tandem mass spectrometry detection. Semen quality was evaluated by measuring volume, concentration, motility, morphology and total sperm count (TSC). Serum samples were analyzed for reproductive hormones, including follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, inhibin B and estradiol. Relationships between urinary BPA concentrations and semen quality parameters and reproductive hormone levels were examined using linear regression, adjusting for potential confounders and covariates. Ninety-five percent of the men had detectable urinary BPA concentrations with unadjusted median (5th–95th) of 2.8 (0.16–11.5) ng/mL. After adjustment for important covariates, there was a significant positive association between urinary BPA concentrations and serum LH levels (β = 0.07, 95%CI: 0.02;0.12, p-value < 0.01). Urinary BPA concentration was also significantly and inversely associated with sperm concentration (β = − 0.04, 95%CI: − 0.07;− 0.02, p-value < 0.01) and TSC (β = − 0.05, 95%CI: − 0.08;− 0.02, p-value < 0.01). No significant associations were found between BPA and other semen parameters or reproductive hormone levels. Our results support the hypothesis that BPA exposure may be associated with a reduction in Leydig cell capacity (increased LH levels) and decreased sperm counts in young men.

双酚A（BPA）是一种普遍的环境毒物，对精子参数和激素水平具有已知的生殖作用。几项观察性研究已经调查了BPA暴露与男性生殖功能之间的关系，但发现并不一致。这项研究的目的是在2010年至2011年间于西班牙南部进行的一项横断面研究中，对215名健康的年轻大学生（18至23岁）进行评估，以评估尿液中BPA浓度与精液质量和生殖激素水平之间的相关性（穆尔西亚地区）。所有受试者在一天中提供尿液，血清和精液样品。尿液中双酚A的浓度通过液-液微萃取和串联质谱检测的超高效液相色谱法进行测定。精液质量通过测量体积，浓度，运动性，形态和总精子数量（TSC）进行评估。分析血清样品中的生殖激素，包括促卵泡激素（FSH），促黄体激素（LH），睾丸激素，抑制素B和雌二醇。使用线性回归，校正潜在的混杂因素和协变量，检查尿液中BPA浓度与精液质量参数和生殖激素水平之间的关系。95％的男性的尿中BPA浓度可检测到，未调整的中位值（第5至95位）为2.8（0.16-11.5）ng / mL。在对重要的协变量进行调整后，尿液中BPA浓度与血清LH水平呈显着正相关（β= 0.07，95％CI：0.02； 0.12，p值<0.01）。尿液中的BPA浓度也与精子浓度（β=-0.04，95％CI：-0.07;-0.02，p值<0.01）和TSC（β=-0.05，95％CI：-0.08;- 0.02，p值<0.01）。在双酚A与其他精液参数或生殖激素水平之间未发现显着关联。我们的结果支持以下假设：双酚A暴露可能与年轻男性Leydig细胞容量减少（LH水平升高）和精子数量减少有关。