In the present study, we report a facile approach to employ gold nanoparticle (AuNPs) and thiol graphene quantum dots (GQD-SH) as the nanomaterial for ultrasensitive detection of streptomycin (STR). Based on this strategy, a GQD-SH was immobilized onto the surface of a glassy carbon electrode (GCE). AuNPs have been immobilized on SH groups of GQDs through bonding formation of AuS and Apt have been loaded on the electrode surface through the interaction between thiol group of aptamer. By incubating STR as a target onto the surface of the prepared Apt/AuNPs/GQD-SH/GCE as a proposed nanoaptasensor, the Apt/STR complex was formed and the changes of the electrochemical signal were evaluated with the EIS technique. The proposed nanoaptasensor showed wide linear range from 0.1 to 700 pg ml^− 1. Finally, the proposed nanoaptasensor was successfully applied for the determination of STR in real samples and satisfactory results were obtained.

在本研究中，我们报告了一种简便的方法来采用金纳米颗粒（AuNPs）和硫醇石墨烯量子点（GQD-SH）作为链霉素（STR）超灵敏检测的纳米材料。基于此策略，将GQD-SH固定在玻璃碳电极（GCE）的表面上。通过Au S的键合形成将AuNPs固定在GQD的SH基团上，并且通过适体的硫醇基团之间的相互作用将Apt负载在电极表面上。通过将STR作为目标物孵育到作为拟议的纳米适体传感器的制备的Apt / AuNPs / GQD-SH / GCE的表面上，形成Apt / STR复合物，并用EIS技术评估电化学信号的变化。拟议的纳米适体传感器显示了从0.1到700 pg ml ^-1的宽线性范围。最终，所提出的纳米适体传感器已成功用于实际样品中STR的测定，并获得了满意的结果。